The Lac Operon 1961, Jacob and Monod E. coli and other bacteria Bacterial Genes Many genes constitutively expressed housekeeping genes Other genes are more ...
... Experiments Defining the Lac-Operon Jacob-Monod Model of Negative Regulation of the Lac-Operon Control of Lac-Operon by CAP and LacI Repressor Leader ...
Promoter is the DNA region where the transcription initiation takes place. ... of his friend and colleague, Melvin Cohn, 'Jacques Monod had one of the most ...
1850's: Gregor Mendel develops 3 'principles' -Segregation -Independent ... 1944: Avery, McCleod and McCarty identify DNA as the 'transforming principle' but...
Operon. genes grouped together with related functions ... single promoter controls transcription of all genes in operon ... Tryptophan operon. What happens when ...
2.) -galactosides initiating biosynthesis at one end of the corridor, ... bacteria initiating biosynthesis. at the other end. Pasteur Institute in France ...
Control of Gene Expression in Bacteria Gene Regulation and Information Flow: an overview Metabolizing Lactose A Model System: multiple genes, classes of mutants ...
if bacterium encounters new sugar (energy source), like lactose, then it needs ... produce enzymes only when nutrient is available. cell avoids making ...
Ampicillin enrichment of lac- cells. One mutant allowed expression in the absence of inducer ... First example of a mutant that affects production not activity ...
QOD What is the lac operon? How is it controlled? Section 11-1 Control of Gene Expression Gene Expression Only some genes are expressed at any given time Cell ...
Title: Tipizzazione ed analisi popolazionistiche dei polimorfismi del locus CYP2D Author: Silvia Last modified by: Thor Created Date: 12/10/2001 2:51:11 PM
Stoichiometric Tumor Model: Phosphorus Flow By: Bo Deng, Vladimir Ufimtsev and Melissa Wilson Funded By NSF/REU Grant to University of Nebraska-Lincoln
Overproduction of Metabolites of Industrial Microorganisms MECHANISMS ENABLING MICROORGANISMS TO AVOID OVERPRODUCTION OF PRIMARY METABOLIC PRODUCTS THROUGH ENZYME ...
... Control of Gene Expression in Prokaryotes Vocabulary ... Anabolic Pathways (e.g. tryptophan synthesis) Product accumulation inhibits the transcription ...
Title: Microbial Genetics Author: Dave Brady Last modified by: Dave Brady Created Date: 3/9/2004 5:35:17 PM Document presentation format: On-screen Show (4:3)
Two ways to Regulate a Metabolic Pathway Control of Metabolism in Prokaryotes and Eukarotes Two major ways to control metabolism Allosteric control of Enzyme activity ...
Expression blocked by a repressor that can be inactivated by a small molecule called an inducer ... to be expressed in inducible fashion with lactose as inducer ...
... in the genetic code would be three nucleotides long Minimum code length that could specify the 20 different amino acids found in proteins This triplet code is ...
Aromatic Amino Acids ... carbon, C-beta branched amino acids contain two (two carbons in ... Role in structure: Alanine is arguably the most boring amino acid. ...
... is the inducer molecule responsible for the increased production of ... The positive control system turn on the expression of the operon ensuring that ...
EVOLUTIONS-teori Tom Sillesen Tak til Naturhistorisk Museum for billeder og inspiration Fra myte til eksperimenter 300 f. Kr. (Platon og Aristoteles tid) : Manden ...
Ligation of variable pieces of either target or vector DNA decreased efficiency ... Directional ligation. ScaI blunt end site. 8/30/09. 22. Final Steps of a ...
... trait (e.g. a fly with white eyes and a fly with vermillion ... Complementation: eye color. Eyes: Red WW, Ww or white ww. Eyes: Red VV, Vv or vermilion vv ...
Gene regulation in Prokaryotes Bacteria were models for working out the basic mechanisms, but eukaryotes are different. Some genes are constitutive, others go from ...
Title: Curs 1 Author: G I Mihalas Last modified by: Mihalas G. Ioan Created Date: 10/1/2003 5:11:15 PM Document presentation format: On-screen Show (4:3)
is required for cloning. Control of Expression. Transcription. turn off (repression) ... Cloning. Restriction Fragment Length Polymorphism. RFLP analysis DNA ...
Lac repressor inhibits transcription by DNA looping ... The lambda repressor binding overlaps a weak -35 region of the PR promoter. The repressor binds to the ...
Historical Landmarks in Our Understanding of Proteins. 1838 The name 'protein' ... to form carbon dioxide and ethanol, thereby laying the foundations of enzymology. ...
Microbiology Tortora. Chapter 0ne- The Microbial World and You ... microbiology ... Golden age of microbiology-began with Pasteur's work. Germ theory of disease ...
Fig. 41-2 Harper's ROB 24th Ed. Positive. Negative. Derepression. Points ... Active vs. inactive chromatin: mCpG islands. Types of Mutations at the DNA Level ...
Translation. Translation- the synthesis of protein from an ... variable in size ~3-~20 nt. tRNA Structure ... (EF-Tu and EF-Ts involved with GTP metabolism in ...
TATA box- where to start. CAAT box and Enhancer- how often ... TATA. Gene. Post-Transcriptional Regulation. 1. mRNA stability can be altered by signal molecules ...
Analyze how a gene can transform an organism and express that gene ... Bacteria present with whitish colonies (regeneration of the starter plate) -pGlo. LB only ...