Bacillus subtilis

1
Associate Professor Liz Harry Institute for the
Biotechnology of Infectious DiseasesUniversity
of Technology, Sydney
The Bacterial Cell Cycle
Bacillus subtilis

• Protein-protein interaction networks using
  Y2H-integration of pathways
• Identification of antimicrobial targets using
  proteomics
• Cell biology-fluorescence microscopy
  immunofluorescence and
• GFP etc fusions- How do cells divide at the
  right time and place?

2
New Discoveries and Challenges
FtsZ ring (YFP)
FtsZ-YFP movie
Immunofluorescence
Need High resolution, high sensitivity, minimal
bleaching 3-D deconvolution Heated stage Smaller
less obtrusive protein label?