Strawberry DNA Extraction

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Strawberry DNA Extraction
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DNA Basics

• Deoxyribonucleic acid
• Genetic material
• Chain of molecules linked together
• DNA contains instructions for traits
• Wrapped tightly around histones
• Forms chromosomes
• Housed in nucleus

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DNA Basics
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DNA Structure

• Double-helix (twisted ladder)
• Sugar and phosphate backbone
• Nitrogen bases
• Subunit nucleotide

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DNA Replication

• Special pairing between nitrogen bases
• New strand is copy of old strand

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DNA Extraction Step 1

• Break the cells open (using force)

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DNA Extraction Step 2

• Dissolve the cell membrane and nuclear envelope
  (using detergent/soap)

Current Protocols in Protein SciencePublished by
John Wiley Sons, Inc.
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DNA Extraction Step 3

• Remove proteins that are bound to DNA (using salt
  to dissolve them)

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DNA Extraction Step 4

• Precipitate DNA (with ethanol)

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DNA Extraction Why?

• Species identification

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DNA Extraction Flow chart
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DNA Extraction Flow chart