Isolating DNA from Fruit

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Isolating DNA from Fruit

• Sherry Adrianos
• Jacque Keele

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Purpose

• Isolation of DNA from fruit using common
  household items and visualizing

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Materials

• 1 tomato, peach, kiwi, strawberry.
• 3 g NaCl salt
• 10 mL clear shampoo
• Water
• 1 liter Zip Lock bag
• 20 mL cold ethanol
• Protease solution
• Coffee filter, knife, beaker, water bath, ice

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Step 1 Cut up fruit
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Step 2- Mix fruit, water, shampoo, and salt
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Step 3- Heat sample for 10 min at 60oC
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Step 4 Place zip lock bag on ice till cool
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Step 5- Filter solution and collect 20 mL of
extract
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Step 6- Add protease solution and cold ethanol
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Fruit DNA Isolated
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Discussion Questions

• What was the purpose of the shampoo?
• What was the purpose of the salt?
• Why was it necessary to mash the fruit?
• Why do you heat and cool the mixture?
• What happens when the cold ethanol is added?
• Would it make a difference if it were warm
  ethanol that was added?

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Acknowledgements

• UW Science Posse
• NIH/NSF Sponsored Science Education Project for
  the State of Wyoming