DNA =

1
DNA - deoxyribonucleic acid - The genetic
material that is located in the nucleus of a
cell. - It contains a code for proteins.
2
Chromosome - an organized structure (or
body) made up of twisted up DNA -
"Chroma" means color - "Some" means body
3
Where have you heard of genes before? What do
genes have to do with DNA? Gene Segments of
DNA that control the production of protein
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DNA and Chromosomes Entrance Ticket
Grade
grade
subject
Subject
date
Date
5
1
Which part of the cell contains DNA?
A
Vacuole
B
Ribosome
C
Cell Membrane
D
Nucleus
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2
The genetic material that contains a code for
proteins is called...
A
RNA
B
DNA
C
Chromosome
D
Gene
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3
When DNA wraps very tightly around itself, it
forms a structure called a(n)...
A
Gene
B
Protein
C
Amino Acid
D
Chromosome
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4
DNA within chromosomes are arranged in segments
called...
A
Genes
B
Proteins
C
Sister Chromatids
D
Amino Acids
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Strawberry DNA Extraction Lab
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DNA deoxyribonucleic acid
"Blueprint of Life"
Guides the construction and development of living
organisms
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(No Transcript)
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Isolate and extract DNA
1. Put cells into special "lysis" solution. 2.
Break cells open (mechanically or
chemically). 3. The ingredients in the lysis
solution remove other parts of the cell away
from the DNA. 4. Filter out extra cell
parts. 5. Add isopropyl alcohol to precipitate
DNA out of a solution
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Lysis Solution
Salt - Na has a charge, so does DNA. The DNA and
the salt are attracted to each other and the salt
forms a "shield" around the DNA and helps it
clump together.
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Lysis Solution
Soap - dishwashing detergent is good at
dissolving grease (which is nonpolar). Like
grease, the nonpolar cellular membranes are
dissolved.
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Why are we using strawberries?
Soft, easy to pulverize Already have enzymes
inside of them breaking down cell
walls Freezing also breaks down cell
walls Strawberries have lots of DNA!
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Procedure
1. Place 1-3 strawberries into a Ziplock Baggie.
Zip closed, gently mash.
2. Add 10 mL of the lysis buffer to the baggie,
zip again and mash until it looks like strawberry
jam. NOTE This solution has soap in it. Do not
eat.
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Procedure
3. Filter the strawberry goo into a beaker using
the coffee filter. You may have to squeeze the
juice through (shown below).
4. Pour the filtered solution into a test tube,
filling it about halfway.
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Procedure
5. Gently add about 10 mL of chilled isopropyl
alcohol to the test tube. Let it sit for a few
minutes until you can see 2 distinct "layers."
6. The white "threads" that you see forming are
strawberry DNA! Wind them up on a wooden stick
and place on black construction paper to get a
better look.
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To Do List for Today
Form groups of 3 Decide the "jobs" that each
person will have - supply collector, observation
recorder, strawberry masher, filterer, etc. Read
procedure and decide what materials your group
will need Have teacher initial this work for
points! Keep packet in your folder for tomorrow.