Leipzig, 23-25 April 2002

1
Garlic and Health, P9 report

• Leipzig, 23-25 April 2002
• INRA, Avignon, France
• V. Chovelon, research scientist
• JP Leroux, research technician
• F. Mirrione, temporary agent (April 02)

2
The objectives

• Somatic embryogenesis
• plant regeneration and acclimatisation
• culture and evaluation
• sanitary aspects
• production of virus-free bulbs
• meristem culture

3
Somatic embryogenesis

• milestones
• Rouge Reunion, Messidrôme, Morasol 200 plants
  from callus and 200 plants from cell suspension
• in vitro culture, acclimatisation
• field culture, evaluation
• flow cytometry (P8), AFLP analyses (P1),
• biochemistry (P5)
• morphological and physiological notations (P8,P9)
• deliverables
• paper on regeneration of garlic plants issued
  from embryogenic callus (done)
• paper on regeneration and acclimatisation of
  garlic plants issued from cell suspension (in
  progress)

4
Rouge Reunion

• plants from callus and cell suspensions

• culture in tunnel
• AFLP analyses (P1)
• flow cytometry
• phenotypic notations
• harvest at maturity

- field evaluation at Reunion Island (P8)
5
In vitro culture
Morasol plants from callus or cell suspension
6
In vitro culture

• Messidrôme plants issued from callus

7
Acclimatisation and field culture

• Morasol plants from callus and cell suspension

8
Acclimatisation and field culture

• Messidrôme plants issued from callus

9
In vitro bulb production

• factors for bulb induction and enlargement

- Morasol, Messidrôme, Printanor plants issued
from cell suspensions - saccharose
concentration 40-60g/l - cold treatment 5 weeks
at 5C - quality of light additional far-red
light - photoperiod 12 or 16h - temperature
22C/night - 24C/day
10
Sanitary aspects

• production of virus-free plants
• 300 plants/variety
• Messidrôme, Morasol
• Printanor, Rouge Reunion
• virological control
• all the plants are potyvirus-free
• some one contained latent viruses
• Messidrôme 100 GCLV, 34 MbFV
• Morasol 5.6 GCLV, 83 MbFV
• Printanor 100 MbFV
• alliin analyses

11
Sanitary aspects

• virus elimination by meristem culture

12
Workplan for 2002-03

• Embryogenesis, genetic characterisation
• Rouge Reunion
• field evaluation (P8), biochemistry (P5)
• Messidrôme, Morasol from callus
• field culture (P9), flow cytometry (P8), AFLP
  (P1)
• Printanor from callus
• in vitro culture, acclimatisation
• Messidrôme, Morasol and Printanor from cell
  suspension
• in vitro bulbs production
• Sanitary aspects
• production of virus-free bulbs (P9)
• meristem culture (P9) acclimatisation, field
  culture