Slayt 1

1
Spa Therapy and Oxidant Antioxidant Systems
2

• Aerobe organisms produce Reactive Oxygen Species
  (ROS)
• Superoxide, hydro peroxide and hydroxyl
• ROS reactions Removing atoms, transferring
  electrons and adding radicals
• Inactivation of enzymes, DNA injury and vital
  injury at cell membrane and decrease cell energy

Introduction
3

• ROS play a major role in events like
• Inflammatory reactions
• Cataract, arteriosclerosis
• Intracellular aging,
• Cell necrosis and apoptosis,
• Ischemia reperfusion injury
• Cancer

Introduction
4

• ROS promoters
• Physical and psychological stress,
• High caloric diet,
• Alcohol and cigarette use
• Air pollution, ozone
• UV light, microwave
• Ionizing radiation
• Some chemotherapeutic
• Hyperthermia treatment also increase ROS
  production

Introduction
5

• Aerobe organisms have antioxidant defence systems
• Intracellular antioxidant enzyme systems
• Super oxide dysmutase (SOD) catalases,
  glutathione peroxides
• In vivo synthesizing substances Bilirubins, sex
  hormones, melatonine, lipoic acide, coenzyme Q,
  and uric acid
• Ceruloplasmin, albumin, transferrin, haptoglobin,
  lactoferrin and hemopexin

Introduction
6

• A, C and E vitamins and
• Plant phenols have antioxidant features
• Oxidative stress may results with adaptation or
  cell damage
• Cells response to mild oxidative stress by
  antioxidant defence systems

Introduction
7

• Oxidative stress
• Damage DNA and other molecules and cause
  degenerative diseases, malignity and aging
• Therapy with hyper thermal baths, drinking cures
  and peloid packs reduce oxidative stress and
  induce antioxidant defence

Introduction
8
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
9

• Patients and Method
• Twenty-eight patients (19 female, 9 male) primary
  osteoarthritis
• Bursa Military Hospital (Spa group)
• Seven patients (5 male, 2 female) Istanbul
  Medical Faculty Medical Ecology Hydroclimatology
  department ambulatory policlinic (Control group)

The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
10

• Spa group patients had 10 15 minute head out
  immersion in 39O-41O C thermomineral water,
  physical therapy and regular exercise for 15 days
  
• Control patients home exercise and simple
  analgesics
• Plasma malondialdehit (MDA) and total antioxidant
  capacity (TAA) were compared

The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
11

• Fasting blood at the beginning (first
  measurement) and thirteenth day (15. Day)
• Plasma MDA and TAA levels in Department of
  Clinical Biochemic by TBARS (Thiobarbituric acid)
  and FRAP (Ferric Reducing Antioxidant Power
  Assay) methods

The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
12
Figure I Spa group, beginning and 15. Day mean
MDA levels (Pgt 0.05)
Statistically insignificant decrease
There was a statistically insignificant decrease
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
13
Figure II Spa group, MDA levels at beginning and
15. Day in patients with beginning MDA
levels are above 6 nmol/ml ( Plt 0.05)
Statistically significant decrease
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
14
Figure III Spa group, MDA levels at beginning
and 15. Day in patients beginning MDA levels
are below 6 nmol/ml ( Plt 0.05)
Statistically significant increase
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
15
Figure IV Spa group the first and15. Day plasma
mean TAA levels ( Pgt 0.05)
No significant difference
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
16
Figure V In spa group male and female the first
and 15. Day plasma mean TAA values. (Plt 0.05)
Statistically significant increase in
men Statistically significant decrease in women
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
17
Figure VII. Control group the first (5.340.65)
and 15. Day (4.640.96) plasma mean MDA values
No significant difference
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
18
Figure VIII. Control group the
first(1065.42143.27) - 15. day (1243.86258.46)
mean plasma TAA values ( Pgt0.05 )
No statistically significant difference
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
19
The effect of spa therapy on plasma
malondialdehit and total antioxidant levels
20
DISCUSSION and
CONCLUSION
21

• Spa therapy decreases oxidant stress ?!
• Increases TAA levels in male and
• Decreases in females (menopause-estrogens
  deficiency?)
• Decrease in oxidant stress is more prominent
• Increase of antioxidant defence is less manifest

DISCUSSION and CONCLUSION
22

• Oxidant effect of hyperthermic baths and
  antioxidant consumption in defence to oxidative
  stress
• Disappearance of inverse proportion between
  disease duration and MDA level
• Balneotherapy more effective in early period of
  disease in decreasing oxidative stress??!

DISCUSSION and CONCLUSION
23

• Decreasing of MDA levels in patients with high
  MDA level
• Increasing of MDA levels in patients with low
  beginning MDA levels
• Regulatory effect of balneotherapy??!!

DISCUSSION and CONCLUSION
24

• Elimination of environmental oxidative factors
• Dietary regime
• Regular exercise
• Physical therapy
• Hyperthermic applications
• Chemical effects of thermo mineral waters

DISCUSSION and CONCLUSION
25
Thanks for your attention