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Copan test (CH ATK)
A short presentation
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a damage
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ANTIMICROBIAL DETECTION
Different analytical methods
Rapid test
chemical
Microbiological test
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Detection of Antimicrobial Substances in the Milk
MICROBIOLOGICAL TEST
broad spectrum of inhibitory substances
MICROBIOLOGICAL TEST
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PREPARATION
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The SPORIFICATION
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The SPORIFICATION
SPORIFICATION ON SOLID
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The PRODUCTION
3 weeks quarantine at room temperature
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The CONTROL
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ANTIMICROBIAL DETECTION
The spores are stimulated to germinate
principally by heating action.
If nutritive substances are present in the medium
the cells could grow and duplicate.
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ANTIMICROBIAL DETECTION
A CROMATIC TEST VERY EASY TO UNDERSTAND
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ANTIMICROBIAL DETECTION
The growing cells produce acid causing the change
of medium pH
The indicator changes to yellow
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ANTIMICROBIAL DETECTION
The cells do not duplicate or do not grow thus
not producing acid
The indicator does not change to yellow
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TESTING LINE

• SINGLE TEST
• 100 TESTS
• 100 NO DROP COUNT PIPETTES

• MICROPLATE
• 4 X 96 TESTS
• ADHESIVES FOR CLOSURE

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HOW TO USE
choose and cut
add milk
incubate
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ANTIMICROBIAL DETECTION

• NO USER SPECIALISATION REQUIRED
• READY TO USE TEST
• EASY TO UNDERSTAND
• EASY MILK DISPENSING SYSTEM
• NO VERY LONG REACTION TIME
• VERY GOOD SENSITIVITY TO SULFAMIDICS AND BETA
  LACTAMS

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ANTIMICROBIAL DETECTION
The detection limits depend on
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EVALUATION STUDIES

• INSTITUTE FÜR HYGIENE UND PRODUKTSICHERHEIT
• BUNDESANSTALT FÜR MILCHFORSCHUNG
• October 2000
• Dr. Getraud Suhren
• MILCHHYGIENE
• LEHRSTUHL FUR HYGIENE UND TECHNOLOGIE DER
  MILCH-TIERARZTLICHE FAKULTAT
• December 2000
• Prof. Martlbauer
• CENTRO DE INVESTIGACIONES TECNOLOGICAS DE LA
  INDUSTRIA LACTES
• ISTiTUTO NACIONAL DE TECNOLOGIA INDUSTRIAL
• May 2000
• Lic. Castaneda

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ANTIMICROBIAL DETECTION
Antimicrobial MRL Copan Test Penicillin G
4ppb ? Ampicillin 4ppb ? Amoxicillin
4ppb ? Cloxacillin 30ppb ? Dicloxacillin
30ppb ? Oxacillin 30ppb ? Nafcillin 30ppb
? Ceftiofur 100ppb ? Cefquinom 20ppb
? Cephapirin 10ppb ? Cephazolin 50ppb
? Cephoperazon ppb ?
beta lactams
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ANTIMICROBIAL DETECTION
Antimicrobial MRL Copan
Test Sulphadoxine 100ppb
? Sulphamethoxazole 100ppb ? Sulphadiazine
100ppb ? Sulphatiazol 100ppb
? Sulphamethazine 100ppb ?
sulfamidics
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Work in progress
8 French laboratories evaluat./compar.
The Food Safety Authority of Ireland
Hannah Institute evaluation
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