Genetic engineering

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Genetic engineering
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• Genetic engineering through selective breeding
• Ex. Dogs, Agricultural products- what else?
• Selective breeding leads to inbreeding mating
  between 2 closely related individuals

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• Selective breeding takes many generations
• Genetic engineering is a faster way of increasing
  a specific gene in a population
• A.K.A recombinant DNA technology

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• Recombinant DNA-recombining fragments of DNA from
  different sources
• DNA from one organism inserted into the DNA of a
  second organism- known as transgenic

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Transgenic organisms- contain genes from other
species

• Tobacco plant with firefly glow gene
• Bacteria plasmids with human genes

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• 1. Isolate DNA to be inserted
• 2. Attach DNA to an insertion vehicle
• 3. Transfer vehicle to host organism

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Restriction Enzymes

• Cut DNA AT SPECIFIC SITES
• Different restriction enzymes cut at different
  places.
• Example EcoR1

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• Use restriction enzyme to cut DNA
• Glue sticky ends together with ligase

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• Extra circular DNA in bacteria
• Move from bacteria to bacteria
• Transformation
• Transfer genetic information

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• Transgenic bacteria
• Contain human genes
• Express human proteins
• Easy and cheap to grow
• Many possibilities

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• Example recombinant DNA Insulin

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Homework

• Read pgs 349-356. Answer Section Assessment
  questions 1-5