Microbial Anatomy

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Microbial Anatomy

• Structural vs Functional Comparisons
• Eucaryotic Cells
• Procaryotic Cells

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The Eucaryotic Cell
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The Procaryotic Cell
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Comparative Functional Anatomy
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Procaryotic Anatomy

• Gross Anatomy
• Size, Shape, Arrangement
• Cell Membranes
• Cell Walls
• Gram positive vs Gram negative
• Intracellular Structures
• Ribosomes, Nucleoid, Spores
• Extracellular Structures
• Flagella, Pili, Capsules

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Bacterial Shapes
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Bacterial Arrangements
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The Membrane
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Phospholipids
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Peptidoglycan
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Gram Positive Cells
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Gram Negative Cells
Gram Negative Cells
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Lipopolysaccharide
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The Gram Stain
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Nucleoid DNA
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70 S Ribosomes
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Flagella
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Chemotaxis
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Bacterial Pili
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Capsules
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Attachment
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Endospores
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Sporulation
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Microbial Nutrition Growth

• Replication Transverse Binary Fission
• Requirements for Growth Physical Temperature,
  pH, Osmotic pressure, O2 Chemical Bulk, Ions,
  Growth Factors
• Culture Media
• Liquid vs solid Complex vs defined
• Aerobic vs anaerobic Selective vs
  differential
• Population Growth
• The growth curve

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Transverse Binary Fission
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Growth Potential
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Water Availability
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Growth
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The Growth Curve
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Blood Agar
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EMB Agar
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Control of Microbial Growth

• History Lister and Semmelwiess
• Terminology
• Physical Methods
• Heat Dry, moist, with pressure
• Radiation Ionizing and non-ionizing
• Chemical Methods Phenols, Halogens, Alcohols,
  Aldehydes, Metals,
• Biguanides, Detergents, Oxides Peroxides

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The Autoclave
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Metabolism

• Introductory Concepts
• Nutritional patterns, Metabolism, ATP, Enzymes,
  Re-Dox reactions, Phosphorylation reactions
• Catabolism
• Glycolysis, TCA cycle, Electron transport chain
• Fermentations
• Ethanol, Lactic acid
• Anabolism
• Biosynthesis

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Nutritional Patterns
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Overview ofMetabolism
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The Flow of Energy
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The Role of ATP
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Enzyme Structure
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Enzyme Action
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Enzyme Function
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Re-Dox Reactions
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Central Metabolism
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Glycolysis
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TCA Cycle (Krebs)
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Energy Trapping
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Electron Transport Chain
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Electron Flow and Energy Trapping
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Fermentations
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Fermentation Products
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Energy Trapping
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EnergyUtilization
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