History

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History

• The Microscope
• Hooke, Leeuwenhoek
• Spontaneous Generation The Cell Theory
• Pasteur, Schleiden, Schwann, Virchow
• Microorganisms and Food
• Apert, Pasteur
• Microorganisms and Disease
• Germ Theory of Disease Koch
• Antisepsis Lister
• Prevention of Disease Jenner
• Treatment of Disease Ehrlich, Fleming

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Contemporary Microbiology

• General Microbiology
• Anatomy, Physiology, Genetics
• Applied Microbiology
• Soil, Aquatic, Food, Industrial
• Medical Microbiology
• Immunology, Virology, Bacteriology, Mycology

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Microbial Diversity

• Bacteria (procaryotes)
• Archaea (procaryotes)
• Eucarya (eucaryotes)
• Viruses (acellular)
• Prions (?)
• A definition of Microbiology

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A Perspective on Size
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Eucaryotes vs. Procaryotes
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Bacteria Procaryotes
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The Eucaryotes
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Protozoa
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Fungi
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Acellular Agents of Disease
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Viruses
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Emerging Diseases
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Principles of Microscopy

• Composition of Microscopes
• Quality of Viewing
• Types of Microscopes
• Staining

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Composition of Microscopes

• Source and Focusing of Illumination
• Specimens and Specimen Holder
• Lens(es) for Magnification

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Brightfield Microscope
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Types of Microscopes

• Brightfield
• Darkfield
• Phase Contrast
• Fluorescence
• Transmission Electron
• Scanning Electron

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Brightfield Microscopy
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Darkfield Microscopy Protista
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Phase Contrast Microscopy Cyanobacteria
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Fluorescence Microscopy
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Transmission EM
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Scanning EM
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Stains

• Simple
• Differential
• Structural
• Electron Microscopy

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Table 03.02
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Stained Bacteria
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Gram Stain
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Acid Fast Stain
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Fluorescent Stains
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Flagella Stain