Prsentation PowerPoint

1
A consensus genetic map in P. vulgaris using a
RILs population constructed from a cross between
an Andean and a Mesoamerican cultivars
2
Identification of a resistance gene family in the
two parents of the RILs population
PRLJ1
LRR
NBS
3
Gene structure of B4 resistance gene analogs
CLUSTER B4
PROTEIN MOTIFS
Nucleotide binding site (NBS)
Leucine Rich repeat (LRR)
Predicted structure of the LRR region
? sheet XX(a)X(a)XX
4
Molecular diversity at the B4 genes family
RFLP
SEQUENCING
PCR
Library
5
Conclusions
-Specialization of C. lindemuthianum on each host
species -Local adaptation of the fungus on P.
vulgaris, no evidence of local adaptation of the
fungus on P. coccineus ? P. coccineus appears as
a good source of resistance for introgression in
P. vulgaris. Durable ?
-Organization of disease resistance genes in
cluster -High level of diversity between the
genes within the cluster B4 -High homology for
resistance gene analogs in the two species ?
development of molecular markers for SAM.