Large Emitting Area Electron Gun

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The 49th ASMS
Large Emitting Area Electron Gun for Electron
Capture Dissociation in Fourier Transform Ion
Cyclotron Resonance Mass Spectrometry
Youri Tsybin, Magnus Palmblad and Per Håkansson
Division of Ion Physics, Ångström
Laboratory, Uppsala University, Box 534, SE-751
21 Uppsala, Sweden
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Slide 2
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What is important for ECD?
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Electron Source
Electron flux up to 10 mA/cm2 optimum 0.5 ?A/cm2
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(No Transcript)
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Improvements
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Substance P (1346 Da)
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Ubiquitin (8,6 kDa)
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Sample concentration 10-5 M Electron pulse
duration 0.1 s Acquisition time 10 min Cathode
potential 0.5 V Filament current 2.2 A Grid
Voltage 5.5 V
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Z223
C10
C242
C243
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C273
C6
C11
C14
C13
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Slide 8
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Integrated ECD and HPLC - FT ICR MS
Peptide mixture
HPLC
FT ICR MS
Write data to disk
FT ICR MS ECD
Write data to disk
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Analysis of peptide mixtures
Session TPL Slot 297
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Conclusions and Outlooks
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Acknowledgments
Oleg Yu. Tsybin (Saint-Petersburg, Russia) Roman
A. Zubarev (Odense, Denmark) Johan Kjellberg,
Jonas Bergquist, Margareta Ramström (Uppsala,
Sweden) sponsors Wallenberg Foundation The
Swedish Research Council