Streptavidin PowerPoint PPT Presentations

To create a fusion protein in E. coli expressing streptavidin on the surface ... functional monomers, Kd = 1.7x10-6 M. Qureshi, 2002: T90A, D128A, Kd = 1.3x10-8 ...

... PP, PS) Surface Plasmon Resonance (SPR) analysis Immunochemistry (rhodamin-labelled streptavidin and streptavidin-labelled gold particles) ...

Sequencing for QuikChange-mutagenized single-chain dimer streptavidin showed ... Received new streptavidin clone (pTSA13) from Dr. Takeshi Sano ...

To offer a new way to target substrates to cells (adaptamer ... anti-thrombin antibody. Adaptamer functionality. Bead surface. adaptamer. thrombin. streptavidin ...

of cyanine 3 tyramide. HRP is inactivated. Streptavidin-HRP binds to. hybridized biotin labeled cDNA. HRP catalyzes deposition. of cyanine 5 tyramide. Schematic IV ...

Protein Micropatterns Using a pH-Responsive Polymer and Light ... on surfaces using a pH-responsive polymer film and a ... Mask. Streptavidin. Scale bar = 25 m ...

To offer a new way to target substrates to cells. ... anti-thrombin antibody. Adaptamer = A15. Control: no thrombin. Adaptamer quenching...

To offer a new way to target substrates to cells. ... To provide a foundation for engineered catalysts. Adaptamer testing. Bead surface. adaptamer ...

Thrombin beads S0 elution. 4. Thrombin beads S0 wash. 3. Thrombin beads T0 elution. 2. Thrombin beads T0 wash. 1. 10bp ladder. L. Condition. Lane ...

Title: PowerPoint Presentation Last modified by: Created Date: 1/1/1601 12:00:00 AM Document presentation format: Other titles

Triggered Sequestration with DNA Nanostructures August 21, 2006 iGEM Week 11: Progress Report Tiffany Chan, Katherine Fifer, Valerie Lau, Matthew Meisel

Welcome to the new era in the detection of radiolabeled compounds. With the potential for continuous, high resolution, multicolor measurements in live cells, aquascint and nanospa enable entirely new measurements, applications, and research insights.Visit: https://bit.ly/43lxwoz

Title: PowerPoint Presentation Author: DuJ Last modified by: kinga Created Date: 4/2/2004 6:41:51 PM Document presentation format: On-screen Show Company

Title: PowerPoint Presentation Author: DuJ Last modified by: kinga Created Date: 6/2/2004 5:44:53 PM Document presentation format: On-screen Show Company

Nuraje, et al. JACS, 2004, 126, 8088-8089. Nuraje, et al. ... Conjugation to BSA. Most common protein in blood. One free cysteine. Carter & Ho, Protein Chem. ...

Cell Surface Targeting

A NOVEL APPROACH TO MAGNETIC FIELD BIOSENSORS: NMR AND SQUID DETECTION A. Valsesiaa, P. Colpoa, F. Rossia, P. Arosiob, M. Marianic,d, M. Cortic, d, M.F. Casulae, A ...

Cell Surface Targeting (we're actually trying it!) Lewis Hahn. Perry Tsai. June 26, 2006 ... Originally used with AIDA-I (adhesin-involved-in-diffuse-adherence) ...

We likewise offer Polystyrene Microspheres 1�m arrangements that contain a lot higher color content than our different items. This permits more grounded signs to be created utilizing less microspheres per following analysis. In numerous organic frameworks, the concentrated fluorescence and round state of the dots grant them to be distinguished against moderately high however diffuse foundation fluorescence.

Cell Surface Targeting contd

No one else got back to us! Autotransporter. Couldn't get strain's genomic DNA ... Change linker sequence, investigate changes in binding affinity ...

Enzyme-Linked ImmunoSorbent Assay ... Anti-analyte Sample + Standard biotin biotin Detection 4. Antibody-biotinilated 3. Sample 2. Blocking 1.

Last Time. Identified two potential problems with our procedure. Use human thrombin, not bovine! ... agarose beads. Design new DNA-DNA interactions to ...

Bad: If we can control amount of aptamer bound to cell surface, probably can get as much/ better control by delivering protein bound directly to the aptamer.

Bio-detection using nanoscale electronic devices Nano-bio interface Nanotube-protein, nonspecific binding Electronic detection in Buffer Environment DNA detection ...

Nanopores: fundamental science

Synthesize polymer that can bind to ketone-modified proteins and can ... P. Mansky, Y. Liu, E. Huang, T. P. Russell and C. Hawker, Science, 1997, 275, 1458-1460 ...

Presence of high molecular weight polymers (e.g., dextran sulfate) ... This is because of the enzyme activity of taq polymerase and Molecular interference ...

Title: PowerPoint Presentation Author: Core lab Last modified by: microarray Created Date: 11/8/2005 10:41:21 PM Document presentation format: On-screen Show

Profacgen provides protein conjugation service on beads to enable protein purification, precipitation, cell isolation, immunoassays and so on. Super paramagnetic beads can quickly gather in the magnetic field, while they can contribute to the separation of dispersed after leaving the magnetic field. Secondly, the particle size is suitable and the difference is small, which ensures that the magnetic response of the magnetic field is strong enough and cannot be settled. 

They are single sheet of carbon atoms rolled together. ... http://www.news.uiuc.edu/NEWS/06/0126nanotubes.html. http://www.sciencemag.org ...

... Binding Protein as a Solubility Enhancer. AND. Gateway Cloning Technology ... Enhances Solubility. Systematic Comparison of. Soluble ... Enhancers ...

Bio-mimetic. Optical. Electrical. Mechanical. DNA. Protein. Glucose. Etc. ... Interactions using bio-mimetic materials (e.g. synthetic bio-receptors). 8 ...

IHC makes it possible to visualize the distribution and localization of specific ... Aliquot antibodies into smaller volumes and store in freezer (-20 to -70?) and ...

Bacterial targeting Fec signal transduction Quorum-sensing luxI/luxR Quorum Sensing Sender + R OHHL Receiver Receivers (luxR + Reporter) GFP Receivers tetR ...

Elution of RseX-bound RNAs. Identification of RseX targets. In vitro transcription of RseX ... Elution of RseX-bound RNAs. Cy3-labelled cDNA synthesis ...

CRP TGTGATCTAGATCACA. CooA TGTCATCTGGCCGACA ... Figure 6. The alignment of recognition motifs of Crp, CooA, and Rv3676-homolog protein. ...

... microarrays Protein expression analysis Drug discovery Clinical diagnostics Others emerging Yeast Proteome Chip First Protein chocolate ... Presentation Last ...

The large circle is the circumference of the GUV. ... From a plot of mean-square displacement against time, the straight line implies ...

... Microfabrication of lab on chip micro arrays of spintronic biosensors Spintronic group at LNESS nanoGune MIC - Copenhagen R. Bertacco Politecnico di Milano ...

Binding Free Energy Calculation Using Molecular Simulations

This is primarily because of their incredible brightness and extensive ... nm and can be functionalized in order to tag nucleic acids, proteins and lipids. ...

Many studies have been done to provide different delivery systems ... E-coli. Mannheimia haemolytica. Actinobacillus pleuropneumoniae. Pasteurella haemolytica ...

Finding new targets with signaling. Random library. Computational Approach ... One-cell/two-cell quorum systems. Characterized. Targeted quorum senders ...

... and Carol A. Parish. Department of Chemistry. Hobart and William Smith Colleges. Geneva, NY 14456 ... Understand how the active site influences the ...

- Aptamers (Steph) - Bacterial Surface Display / Fusion Prot. (Perry) - Target Bact to certain tissues (actuator or product) - Targeting Microbial Factories

Genom-screening med Illumina SNP-array Beskrivelse af beadchippen Princippet bag detektion af variationer med SNP-array SNP-array fremgangsm de

Transducer on left (pipette 1) RBC treated, with biotin on surface, in hypotonic buffer ... Target on right (pipette 2) WBC at room temperature in chamber ...

Microscopy Special Techniques in Microscopy: Immunohistochemistry Immunohistochemistry The process for detecting antigens (Ags) in histological material uses ...

Microcontact Printing Yes Yes No No No AFM Mechanical Scribing and Nanoindending Overview Yes Yes No No No c-AFM Oxidation A B APDES Nanografted onto SiO2

HER2/neu gene is a proto-oncogene located on 17q21 and ... CA-125, serous cystadenoma of ovary. Calretinin, mesothelioma. limfoiduri infiltracia kuWSi, H&E ...

Western technique analyses proteins after gel fractionation and ... Increases elution of SDS from the gel. Increases binding of proteins to the membrane ...

Chapter 6 Ag-Ab Interactions: Principles & Applications Fluorescent Ab staining reveals intracellular Ig Nov 2, 2006 Chapter 5 Ab Engineering (p. 136 142) Human ...

Modulation of Conductance in a Carbon Nanotube Field Effect Transistor by Electrochemical Gating - Application to the detection of unique sequences of DNA

Initial targeting was done by displaying histidine and strep2 tags on the E. ... An aliquot from the RFP-sender MACS elution was dropped in the center of the plate. ...

Title: No Slide Title Author: Paul Alivisatos Last modified by: SC Created Date: 10/12/2000 9:22:41 PM Document presentation format: On-screen Show