Microscopes

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Microscopes
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What is this?
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(No Transcript)
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I. Parts of the Compound Light Microscope

• Metal or plastic part on which microscope rests
• BASE

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Somewhat C-shaped pillar arising from the base
and supports the stage and ocular components.
Used for carrying

• ARM

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Flat platform attached to lower portion of arm on
which slides or samples are

• STAGE

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Collects light rays and focuses them on the
specimen slide. Can be adjusted for better
resolution (sharper image)

• DIAPHRAGM

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Convex lens used to magnify the image generally
10-15x magnification

• Ocular or eyepiece

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Small convex lenses screwed into nosepiece and
increase the magnification of the sample.

• Low power 4x
• high power 40x
• OBJECTIVES

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Larger knob which moves the base rapidly up or
down to get the sample into coarse focus

• COARSE ADJUSTMENT KNOB

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Smaller knob which moves the base slowly through
short distances to get the sample into sharp focus

• FINE ADJUSTMENT KNOB

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REMEMBER

• All specimens must be thin for LIGHT to shine
  through them so that you can see clearly.

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REMEMBER

• Only use the FINE ADJUSTMENT KNOB when focusing
  on medium or high power objectives!!
• This avoids damaging or breaking the slide

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Electron microscope

• Electron microscope a microscope that focuses
  beams of electrons instead of light rays.
  Produces clearer, 3D images.

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Muscle Tissue!
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LIGHT MICROSCOPE VS. ELECTRON MICROSCOPE
LIGHT MICROSCOPE ELECTRON MICROSCOPE
I. USED TO VIEW LIVING OBJECTS I. OBJECTS CANNOT BE ALIVE
II. COLOR AND MOVEMENT CAN BE OBSERVED II. BLACK AND WHITE IMAGES WITH NO MOVEMENT
III. POOR RESOLUTION III. EXCELLENT RESOLUTION
IV. EASY TO USE/INEXPENSIVE IV. VIEWER MUST BE TRAINED/VERY EXPENSIVE
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Calculating magnification

• Just multiply the objective lens power by the
  ocular lens power
• Lower power 10 X 4 40
• Medium power 10 x10 100
• High power 10x40400

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Guided Practice

• Instructions
• You will be using your fingers to show me the
  correct answer for the following questions
• We are going to read the question SILENTLY. DO
  NOT CALL OUT THE ANSWER.
• Think about the question and pick an answer.
• When I count to 3, You are going to hold up your
  choice.
• DO NOT LOOK AT ANYONE ELSES ANSWER!! THIS IS
  YOUR TIME TO FIGURE OUT IF YOU KNOW THE
  INFORMATION!

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QUESTION 1

• 1. One advantage of using a light microscope is
  that it
• A. has high resolution
• B. magnifies clearly to 1000x
• C. can be used to view living cells
• D. can be used to view atoms

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QUESTION 1

• 1. One advantage of using a light microscope is
  that it
• A. has high resolution
• B. magnifies clearly to 1000x
• C. can be used to view living cells
• D. can be used to view atoms

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QUESTION 2

• 2. When you are focusing on medium or high power
  objectives, you should use the
• Coarse knob adjustment
• Fine knob adjustment
• Ocular
• Diaphargm

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QUESTION 2

• 2. When you are focusing on medium or high power
  objectives, you should use the
• Coarse knob adjustment
• Fine knob adjustment
• Ocular
• Diaphargm

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Question 3

• The purpose of the diaphragm is to
• Adjust the amount of light focusing on the
  specimen
• Magnify the specimen on the stage
• Adjust the magnification of the specimen
• View the specimen on the stage

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Question 3

• The purpose of the diaphragm is to
• Adjust the amount of light focusing on the
  specimen
• Magnify the specimen on the stage
• Adjust the magnification of the specimen
• View the specimen on the stage

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Question 4

• If a microscopes ocular is marked 10x and the
  low power objective is marked 4x, what would be
  the total magnification when using the low power
  objective?
• A. 10x C. 40x
• B. 12x D. 400x

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Question 4

• If a microscopes ocular is marked 10x and the
  low power objective is marked 4x, what would be
  the total magnification when using the low power
  objective?
• A. 10x C. 40x
• B. 12x D. 400x

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Question 5

• If a microscopes ocular is marked 10x and the
  low power objective is marked 30x, what would be
  the total magnification when using the low power
  objective?
• 3x
• 300x
• 30x
• 3000x

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Question 5

• If a microscopes ocular is marked 10x and the
  low power objective is marked 30x, what would be
  the total magnification when using the low power
  objective?
• 3x
• 300x
• 30x
• 3000x

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Question 6

• One disadvantage of using an electron microscope
  is that it
• A. has high resolution
• B. Very expensive
• C. shows details of the surface
• D. can be used to view atoms

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Question 6

• One disadvantage of using an electron microscope
  is that it
• A. has high resolution
• B. Very expensive
• C. shows details of the surface
• D. can be used to view atoms

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(No Transcript)
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Worktime

1. How should you carry a microscope?
2. What knob should you use to focus when using a
   high power objective?
3. Why should you not use the coarse knob adjustment
   when using the high power objective?

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Worktime

1. How should you carry a microscope?
2. What knob should you use to focus when using a
   high power objective?
3. Why should you not use the coarse knob adjustment
   when using the high power objective?
4. What is the purpose of the diaphragm?
5. Where is the ocular lens located?
6. Where are the objective lens located
7. What is the purpose of the stage?
8. If the ocular lens has a magnification of 10x and
   the objective lens has a magnification of 25x,
   what is the total magnification?
9. If the ocular lens has a magnification of 4x and
   the objective lens has a magnification of 50x,
   what is the total magnification?
10. If the ocular lens has a magnification of 5x and
    the objective lens has a magnification of 100x,
    what is the total magnification?

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Worktime

1. How should you carry a microscope?
2. What knob should you use to focus when using a
   high power objective?
3. Why should you not use the coarse knob adjustment
   when using the high power objective?
4. What is the purpose of the diaphragm?
5. Where is the ocular lens located?
6. Where are the objective lens located
7. What is the purpose of the stage?
8. If the ocular lens has a magnification of 10x and
   the objective lens has a magnification of 25x,
   what is the total magnification?
9. If the ocular lens has a magnification of 4x and
   the objective lens has a magnification of 50x,
   what is the total magnification?
10. If the ocular lens has a magnification of 5x and
    the objective lens has a magnification of 100x,
    what is the total magnification?

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Exit Ticket1

• I used to work at an Air force base. We would
  work with particles of metal called nano
  particles. The particles were dead, and
  microscopic. What would be the best way to see
  those?
• A light microscope
• An electron scanning microscope
• A light telescope
• An electron scanning teloscope

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Exit Ticket 2

• 2) When the low power objective is being used you
  should adjust it using the
• a) fine adjustment knob
• b) diaphragm
• c) course adjustment knob
• d) ocular adjustment knob

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Exit Ticket 3

• 3) Which of the following statements is NOT true
  about objects viewed with an electron scanning
  microscope?
• A. They must be thin enough to allow light to
  pass through them.
• B. They must be living specimens
• C. No dyes or states can be used on the objects
• D. They must be made of organic material

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Exit Ticket 4

• 4) If the microscopes ocular lens is marked 10x
  and the lower power objective is marked 5x, what
  is the total magnification?
• a) 10
• b) 5
• c) 50
• d) 500

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Exit Ticket 5

• 5) What is the proper way to place a cover slip
  on a wet mount slide?
• a) c)
• b) d)