Microscopes

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Microscopes

• Day 1

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What is a Microscope?

• Microscope an instrument that produces
  larger-than-life images

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What does a Microscope do?

• A microscope has 2 jobs
• Magnification to make something appear larger
• Resolution the ability to distinguish two
  objects as being separate from each other

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More about resolution

• Resolution, not magnification, increases the
  amount of detail you can see in an object!

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Good Resolution Bad Resolution
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Magnification Resolution

• As magnification INCREASES, resolution DECERASES

Magnification
Resolution
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Compound Microscopes

• We will be using a Compound Light Microscope a
  microscope that uses glass lenses that focus
  light passing through an object

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Parts of a Compound Microscope

• Eyepiece contains the ocular lens you look
  through

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Parts of a Compound Microscope

• Arm supports the eyepiece and serves as a
  handle

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Parts of a Compound Microscope

• Objective Lenses name for the lenses located
  between the eyepiece and stage

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Parts of a Compound Microscope

• Stage platform used to support the microscope
  slide

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Parts of a Compound Microscope

• Diaphragm regulates the amount of light that
  passes through an object

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Parts of a Compound Microscope

• Light Source allows light to reflect upward
  though the diaphragm, specimen, and lenses

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Parts of a Compound Microscope

• Base- supports the entire microscope

Base
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Parts of a Compound Microscope

• Coarse Adjustment Knob focuses the image under
  low power

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Parts of a Compound Microscope

• Fine Adjustment Knob perfects the focus of an
  image

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Calculating Magnification

• Total Magnification how much larger an object
  appears when viewed through a microscope

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The Letter X

• X stands for how many times a particular lens
  magnifies an object
• Ex 10X object is magnified 10 times

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Easy Math ?

• FORMULA
• Total magnification (magnification of eyepiece)
  x (magnification of objective)

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Example

• What is the magnification if?
• eyepiece is 10X
• low power objective is 10x

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The answer is

• (10X) x (10X) 100X

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• Were DONE!
• ?

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Microscopes

• Day 2

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All Kinds of Microscopes

• Lets talk about the gobs of microscopes that
  existyay ?

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Dissecting Microscope

• Dissecting Microscopes produce 3-D images of the
  surface of the object being viewed

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Dissecting Microscope

• Typically have 2 eyepieces
• cannot magnify as much as a compound microscope
• good for viewing live specimens up close

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Electron Microscopes

• Electron Microscopes use a beam of electrons to
  illuminate the object being viewed
• There are 2 kinds of electron microscopes

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Transmission Electron Microscope (TEM)

• Transmission Electron Microscope (TEM) - passes
  an electron beam through very thin sections to
  study the internal structure of specimens
• Produces a 2-D image

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Wickedly Awesome Things About TEM

• sample is embedded in plastic and sliced with a
  laser into thin sections
• uses electromagnets instead of lenses to focus
  objects
• image is seen on a screen or as a photograph
• Can magnify things 50 million times (holy cow!)

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WoahTEM atoms!
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Scanning Electron Microscope (SEM)

• Scanning Electron Microscope (SEM) uses an
  electron beam to scan the surface of a sample to
  study details of is exterior

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Neat Things About SEM

• produces a 3-D image
• specimen is coated with a thin layer of gold so
  electrons can bounce off of it
• image is seen on a TV-like screen

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