Identifica

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Identificação de leveduras

• Taxonomia clássica

2
IDENTIFICAÇÃO GENÉRICA
Microcultivo Agar fubá Tween 80 Corn meal agar
Extrato de malte
Meio de acetato Gorodkowa YM Água de torneira

• Outras provas
• Urease
• Cápsula
• Meio DBB

Macroscopia Sedimento Resíduo Anel Ilhota Película
Microscopia Forma e tamanho das células
Microscopia Produção esporos sexuais Ascos e
ascósporos
Microscopia Pseudomicélio, Micélio
verdadeiro, Balistósporos Clamidósporos
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IDENTIFICAÇÃO ESPECÍFICA
Zimograma Fermentação Alcoólica de açúcares
Auxanograma
Meios diferenciais -meio TTC -ágar
molibdênio -ágar azul Tripan -meio de
Nickerson -meio de CGB

• Outras provas
• Crescimento 37ºC
• Produção de
• compostos amilóides
• -tubo germinativo
• -resistência a
• Cicloheximida
• -fenol oxidase

Assimilação Fontes C e N
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The xylose-fermenting yeast, Pichia stipitis (A)
during vegetative growth, (B) forming spores, and
(C) under carbon limitation. (FPL photo-Tom
Kuster)  
The xylose-fermenting yeast, Pichia stipitis (A)
during vegetative growth, (B) forming spores, and
(C) under carbon limitation. (FPL photo-Tom
Kuster)  


2006 Highlights

   
2006 Highlights

   
o How Well Do Arsenic-Free Preservatives Inhibit Copper-Tolerant Fungi?
o Caulking with Care
o Combustion Properties of an Exotic Annual Grass
o Creosote Movement from Treated Wood Immersed in Fresh Water
o Durable Wood, Naturally--Termite Resistance
o Essential Oils Inhibit Mold Spore Germination
o Fire Resistance of Strucutral Composite Lumber Products
o Bioprocessing for Ethanol Production and a Valuable Adhesive Coproduct
o Fuel Treatment Evaluator
o Fuel Treatment Market
o Genome Sequencing
o Wood Research from World War I to Iraq
o Reusing Remediated Wood in Value-Added Products
o Inspecting Historic Structures Using the Web to Train Inspection Professionals
o Productive Use of Thinnings in Dimension Lumber and Paper
o Treatability of Underutilized Wood Species
o Enhancing Wood-Plastic Composites by Crosslinking Polymers
o Accessible and Affordable Playground and Path Surfacing Now Commerically Available
o Dimensional Warping of Wood-Based Composites
o How Well Do Arsenic-Free Preservatives Inhibit Copper-Tolerant Fungi?
o Caulking with Care
o Combustion Properties of an Exotic Annual Grass
o Creosote Movement from Treated Wood Immersed in Fresh Water
o Durable Wood, Naturally--Termite Resistance
o Essential Oils Inhibit Mold Spore Germination
o Fire Resistance of Strucutral Composite Lumber Products
o Bioprocessing for Ethanol Production and a Valuable Adhesive Coproduct
o Fuel Treatment Evaluator
o Fuel Treatment Market
o Genome Sequencing
o Wood Research from World War I to Iraq
o Reusing Remediated Wood in Value-Added Products
o Inspecting Historic Structures Using the Web to Train Inspection Professionals
o Productive Use of Thinnings in Dimension Lumber and Paper
o Treatability of Underutilized Wood Species
o Enhancing Wood-Plastic Composites by Crosslinking Polymers
o Accessible and Affordable Playground and Path Surfacing Now Commerically Available
o Dimensional Warping of Wood-Based Composites
The xylose-fermenting yeast, Pichia stipitis (A)
during vegetative growth, (B) forming spores, and
(C) under carbon limitation. (FPL photo-Tom
Kuster)
2006 Research Highlights
2006 Research Highlights
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A. Haploid yeast cells buddingB. Haploid cells
forming shmoos and zygotesC. Zygote budding off
diploidD. Diploid buddingE. Diploid forming
asci with ascospores freed haploid spores
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Teste do DBB. As linhagens CCA 181, 506 e 567
são DBB negativas, enquanto a CCA379 é DBB
positiva (utilizada como controle positivo)
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ESPOROS SEXUAIS
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Produção de pseudomicélio
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Candida albicans - pseudomicélio
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Classificação das leveduras

• Reino Fungi
• Divisão Eumycota
• Subdivisão Ascomycotina
• Subdivisão Basidiomycotina
• Subdivisão Deuteromycotina

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Chaves de identificação

• Kreger-van Rij, N.J.W. The yeasts. A taxonomic
  study. Elsevier, 1984.
• Barnett, J.A., Payne, R.W., Yarrow, D. Yeasts
  characteristics and identification. Cambridge
  Univ. Press, 1990.