Benjamin Cummings

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Gerald Filipski, University of Alberta
Benjamin Cummings
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Univ. Illinois
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King, Summers, Haefemeyer, LeRoy
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Parts of a Gene
Gene
coding region
promoter
terminator
Upstream of gene
downstream of gene
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Transcription Nucleic Acid (DNA) to Nucleic
Acid (RNA)
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Promoter Regions
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Prokaryotes
Pribnow Box
-10
-35
TATAAT
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TTGACA
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Eukaryotes
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CAAT Box (variable)
TATA Box
-25
-75
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TATAAA
GGNCAATCT
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Oak Ridge National Laboratory
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Dave Ussery
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Benjamin Cummings
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mRNA
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CCAGAUGCGCAAGUCCGGGGACCCCUUCGAAAAUCCGGUAUGAGGCACG
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Initiation of Translation
Pearson Education
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Elongation and Termination of Translation
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Wiley
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Addison Wesley
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Griffiths et al. 2004
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Biocrawler.com
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Protein that activates the copying of HIV
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National Human Genome Research Institute
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Differences between prokaryotic eukaryotic
protein synthesis
Prokaryotes
Eukaryotes
Only 1 RNA polymerase used in transcription
Three different types of RNA polymerases used in
transcription
Transcription and translation occur simultaneously
Transcription and translation separated in time
and space
mRNA is not modified
mRNA is modified
proteins are not modified
proteins are modified
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3 CGTACCTTGAGTTATACCGCTCAATGGGGCAAACTTC 5
mRNA