Geen diatitel

1
bp
2
Lanes indicated with M molecular size markers
(100 base pair ladder (Pharmacia Biotech,
Uppsala, Sweden). Figure 1. ARDRA patterns
obtained after restriction of the amplified 16S
rDNA with CfoI. 1 pattern 1, Corynebacterium
afermentans subspecies afermentans (CIP
103499T) 2 pattern 2, CDC coryneform group ANF
1-like (IBS T30014) 3 pattern 3, C. minutissimum
(ATCC 10288T) 4 pattern 4, C. urealyticum (ATCC
43042T) 5 pattern 4, CDC coryneform group ANF
3-like (IBS T88848) 6 pattern 5, C. amycolatum
(CIP 103452) 7 pattern 6, C. pseudotuberculosis
bv. equi (CIP 5297) 8 pattern 7, CDC coryneform
group B-1 (IBS T94631) 9 pattern 8, CDC
coryneform group B-3 (IBS T95303) (Pattern 9
(coryneform group B-3 (IBS T79396) not
shown) Figure 2. ARDRA patterns obtained after
restriction of the amplified 16S rDNA with AluI.
Lane numbers correspond with pattern numbers
1 Corynebacterium afermentans subspecies
afermentans (CIP 103499T) 2 C. afermentans
subsp. lipophilum (CIP 103500T) 3 C. diphtheriae
(CIP 100721T) 4 C. jeikeium (CIP 103337T) 5 C.
pilosum (ATCC 29592T) 6 C. propinquum (CIP
103792T) 7 C. cystitidis (CIP 6940) 8 'C.
macginleyi' (IBS B72932) 9 CDC coryneform group
F-1, genomospecies 1 (IBS B1657) 10 C.
pseudotuberculosis bv. ovis (CIP 102968T) 11 C.
renale (CIP 6937) 12 CDC coryneform group
ANF3-like (IBS T88848) 13 CDC coryneform group
B-1 (IBS T94631) 14 CDC coryneform group B-3
(IBS T79396) 15 Rhodococcus equi (CIP
5472T). Figure 3. ARDRA patterns obtained after
restriction of the amplified 16S rDNA with RsaI.
1 pattern 1, C. afermentans subsp. lipophilum
(CIP 103500T) 2 pattern 2a, C. diphtheriae (CIP
A 102) 3 pattern 2b, C. pseudotuberculosis bv.
equi (CIP 5297) 4 pattern 2c, C.
pseudotuberculosis bv. ovis (CIP 102968T) 5
pattern 3, C. propinquum (IBS B50121) 6 pattern
4, C. striatum (CDC E 8873) 7 pattern 5, C.
urealyticum (ATCC 43043) 8 pattern 6, C. xerosis
(IAM 12431) 9 pattern 7, CDC coryneform group G,
genomospecies 1 (LCDC 81613) 10 pattern 8, CDC
coryneform group F-1, genomospecies 1 (IBS
B1657) 11 pattern 9, CDC coryneform group ANF
3-like (IBS T88848) 12 pattern 10, C. renale
(CIP 103421T) 13 pattern 11, CDC coryneform
group B-3 (IBS T95303) 14 pattern 12, CDC
coryneform group B-3 (IBS T79396) 15 pattern 13,
Rhodococcus equi (CIP 5472T)
3
ARDRA identification scheme of coryneforms