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Dr Derakhshandeh Transposons Mobile Genetic Elements Transposons or Transposable elements (TEs) move around the genome Transposable elements in prokaryotes Insertion ...
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Gene Expression Transcription Factors RNA Splicing Recombinant DNA Proteasome DNA Testing Practical Application Human Genome Project Safety and Ethical Questions ...
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Transposons. First discovered in plants. Transposition to a new site ... Class I : Retrotransposons. Class II: Transposons. Class III: Miniature Inverted ...
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Examples of composite transposons Formation of a composite transposon Selection of Transposing sites can result in new transposons Transposon site selection Can lead ...
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Able to move from one place to another within a cell's genome ... Ligation of transposon into target site. DNA gaps filled by DNA polymerase. TRANSPOSONS ...
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Species, Tissue and Position along a chromosome ... It recruits protein complex that induce the alternation of the local chromosome structure ...
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Regulation of MuDRMu Transposons of Maize
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The Nature of Transposons Chapter 11 pp 300-315 Outline Nature of Transposons Transposons Prokaryotic Eukaryotic: Dr. McClintock s research Retrotransposons vs ...
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Signals, Development, Transposons
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This pathway should be kept in the 'off' state when lactose is absent. When lactose is present, the bacteria can burn it for fuel. ...
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Variegation in petal color due to activity of a transposon at the W4 locus ...
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Reproduction Specific Argonaute Genes In Maize and Barley And Their Role In Transposon Silencing Manjit Singh1, Daniel Grimanelli2 and Jaswinder Singh1
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... alignments tend to break at transposon insertions, inversions, duplications, etc. ... Alignments are interrupted by numerous recent transposon insertions. ...
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3 trans-acting genes: 2 cis-acting sites: 38 bp inverted repeat ends ... usually with sister chromosome, restoring transposon at original site ...
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... Sequences (IS elements) Composite transposons. Tn3 elements. IS ... DS is a truncated copy of AC that is non-autonomous, requiring AC in order to transpose ...
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Transposable elements: DNA sequences that are inserted into new locations Transposon is a longer transposable element (5,000 base pairs) ...
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May originally have been transposons Conferred a benefit Became able to replicate autonomously Acquired genes to form a protein coat Mitochondria and chloroplasts ...
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Cell culture. sexual life cycles in all kingdoms. Transposons. By: Vanessa Ghofrani, Cheyenne Cheung, Cody Rubin ...
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Replicative transposition. Transposon is replicated. Donor retains a copy of transposon ... Promotes recombination within the same DNA molecule. Recognizes ...
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The transposase first cleaves one DNA strand at each end of the transposon, ... inserts the transposon into target site using the DNA strand transfer reaction. ...
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Aliens? Oddities? Or misunderstood? Transposons and miRNAs Genome sizes (haploid) Number of genes in different organisms What is a transposon? Contiguous piece of DNA ...
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Terminology Genetics Study of what genes are how they ... Complex transposons carry other genes Regulation of Bacterial Gene Expression Constitutive enzymes ...
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Mechanisms of resistance Lab detection Epidemiology Treatment Infection control What works? MRSA mec determinant 30 kb transposon mec gene approx 2.5 kb on ...
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... of a Pseudomonas aeruginosa transposon mutant disrupted in the ... 7 kb genetic element ... factor for human opportunistic pathogen Pseudomonas ...
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... transposons Resistance to Antibiotics Antimicrobial peptides Broad-spectrum antibiotics Nisin (lactic acid bacteria) Magainin (frogs) Cecropin (moths) ...
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... DNA transposons TNs Transpose through DNA no RNA intermediate Contain inverted repeat sequences at end Autonomous TNs encode transposase Mechanims of ...
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Transposons ADN et g nes domestiqu s Ludivine SINZELLE Post-doc, Laboratoire Epigenomics G nopole, Evry Il existe 2 classes d ETs d finis par le m canisme ...
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a 'rough draft' (published 2001 in Science and Nature) of the base sequences of ... makes tracing ancestry trickier. Junk DNA? Hypervariable. because of transposons ...
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Sequencing - Marine bacillus, carotenoids, proteorhodopsin repot. Dave Ward - cyano. George Fox - transposon bioinformatics (see attached ) ...
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Conserved pathway critical to the stability of genome. Implicated in silencing of transposons, ... Single nucleotide changes in siRNAs abrogate interference. ...
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Gene Inactivation Michael Snyder October 2, 2006 Problems No phenotype Effects might be indirect Three General Methods 1) Insertional Mutagenesis Transposon ...
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Transposons: single inserts at random position (drosophila, yeast, E.coli) ... Abstrait. General. transposable. Mod les de paysage' fitness. Trait 1. Trait 2. fitness ...
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MER121: The most interesting 'junk' in the genome. Ancient Repeats: Fossils of Dead Transposons ... Ancient 'junk' still present in genomes: ~22% of human ...
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Most is repetitive DNA Transposable elements Almost half the human genome Unique noncoding; pseudogenes Transposons DNA intermediates May be excised and moved, ...
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Transitions:a kind of the simplest mutations which are pyrimidine-to-pyrimidine ... by transposon or by aberrant action of cellular recombination processes. ...
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... consisting of two insertion sequences and one or more intervening genes. ... transposon inserted in gene, abnormal RNA, abnormal protein, loss of function. ...
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neomycin. MCS. SmaI. XmaI. SalI. BbvII. DraIII. EcoO109 1. PpuMI. BamHI. BspM II. PflM I. See Manoil, C. and Beckwith, J. (1985) TnphoA: A transposon probe for ...
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Select for a phenotype and recover the gene from bacterial or phage clone ... Positional Cloning. ... This method works also for transposon based cloning...
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I will assign reading for each of the topics, starting with imprinting for ... Agouti mice: transposon inserted near agouti gene alters methylation leading to ...
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Genetic variation is essential for evolution and change to occur ... If a transposon jumps in some cells but not others, can get a variegated phenotype ...
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Les m canismes de transfert Chez les Bact ries M canismes passifs La transformation M canismes actifs La transduction La conjugaison Chez les Eucaryotes
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Title: Slide 1 Author: jrawls Last modified by: DOUGH Created Date: 1/26/2005 8:30:03 PM Document presentation format: On-screen Show (4:3) Company
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MuDrA and B expressed at ... Arial Times New Roman Calibri Default Design Slide 1 Slide 2 Genome Instability and Repair Slide 4 Other characteristics of ...
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Transposition Evidence Mechanisms: DNA-mediated RNA-mediated
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Evolution and Ecology of Antibiotic Resistance Background Time scale Evolution of pathogenesis: millions of years Evolution of a/b resistance: 60 years?
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How do individuals and groups with different genes arise? Exams returned W 2/17? No discussions 2/15 or 16 DNA Composition: In humans: Each cell contains ~6 billion ...
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... oligonucleotide with the corresponding change in association with the DNA ... of the protein into a living organism can be used to assess the in vivo function ...
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All types of mobile genetic elements occasionally move or ... The best example of the conservative site-specific recombination is bacteriophage lambda. ...
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Prokaryotic Gene Expression II Bacteria Overall outline Introduction to Bacteria Genetic Recombination in Bacteria Regulation of Transcription Introduction to ...
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Transposable Genetic Elements Transposable Genetic Elements P elements in Drosophila - 2.9kb with 31 base inverted repeats encodes a transposase - Do not utilize an ...
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Title: Life: The Science of Biology, 8e Author: maria luisa Last modified by: Sara Urbani Created Date: 10/16/2000 7:08:56 PM Document presentation format
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Research Objective. investigate the position of nucleosomes in Arabidopsis thaliana. study the relationshi p of DNA methylation with nucleosome. positions
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Gene Therapy Gene therapy: the attempt to cure an underlying genetic problem by insertion of a correct copy of a gene. Tantalizingly simple and profound in theory ...
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Bacterial Genetics By Dr. Emad AbdElhameed Morad Lecturer of Medical Microbiology and Immunology Plasmids Extra-chromosomal circular double stranded DNA molecules.
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MGH-PGA. Genomic Analysis of Stress and Inflammation: Pseudomonas aeruginosa Strain PA14 Non-Redundant Mutant Library ... (http://pga.mgh.harvard.edu/cgi-bin ...
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The amount of crossing over is minimal and only occurs ... Hybrid Dysgenesis. P-elements with hybrid dysgenesis serve as transformation vectors and as genetic ...
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