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Title: M todos de Isolamento de Biomol culas Author: Celia Carlini Last modified by: usuario Created Date: 3/25/2000 1:05:34 AM Document presentation format
Proteolytic peptides with masses above 3000 Da are of interest because they are ... carried out at 140oC in 12 % acetic acid for 20 min. Peptides were fractionated ...
... de novo de prote nas por espectrometria de massas Para sequenciar prote nas preciso obter o espectro MS/MS de ... FTICR Consiste de tr s pratos ...
Using Proteomics for Biomedical Research Matrix for MALDI ToF 2,5-dihydroxybenzoic acid (DHB) -cyano-4-hydroxy-cinnamic acid 3,5-dimethoxy-4-hydroxycinnamic acid ...
fax = ( 2qVtrap/md2)1/2. d = distance between trapping plates (1% term) ... SIMS: Secondary Ion Mass Spec. Ion beams: easy to create, but charges the ...
Large-scale phosphorylation site mapping ... Immobilized metal affinity chromatography (IMAC) ... Introduce affinity tag to enrich for phosphorylated molecules ...
compared specimens of human serum and EDTA, heparin, and citrate-anticoagulated plasma ... Tissue- minced in PBS- ms- secretome. Session: post-tran modification ...
PC s UNIX Mac Different Types of Mass Analyzers Magnetic Sector Analyzer (MSA) ... Genomics - A field of life science research that uses High Throughput ...
They may be one analyzer operating in two different modes ... reasons it is used in special problems. fragmentation ... The 20 amino acids and their properties ...
Resolving power defines how well the mass spectrometer can ... Off-axis skimmers and ring lens, bent ion guide. Keep contamination out of high-vacuum region ...
Quaternary Structure of the Collagen IV 1256 Hexamer Wes Robertson Mentors: Dr. Billy Hudson, Dr. Roberto Vanacore BSCI 296 Combined manual with bioinformatics ...
Chemical Analysis by Mass Spectrometry Dr Phil Mortimer Chemistry Department Mass Spectrometry Facility 410-516-5552 mass.spec@jhu.edu Mass spectrometry MALDI ...
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