ProVision 360

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ProVision 360
Commercialized Version of Explosives Detection
Personnel Portal with IMS Detection
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Mass spectrometers at the Institute of
Microbiology, Prague, Dec 1, 2006
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Mass Spectrometer
Normal pressure
Reduced pressure
Sample introduction
Mass Analyzer
Computer

• Sensitivity
• Specificity
• Speed
• Simplicity of data interpretation

Well recognized unique attributes of Mass
Spectrometry
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DESORPCNÍ TECHNIKY

• Desorpce
• polem (Beckey)
• 1974 Desorpce
• plazmatem 252Cf
• (McFarlane)
• Ionizace
• rychlými atomy
• (Barber)
• Elektrosprej
• (Fenn)

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DESORPCNÍ TECHNIKY

• Desorpce
• polem (Beckey)
• 1974 Desorpce
• plazmatem 252Cf
• (McFarlane)
• Ionizace
• rychlými atomy
• (Barber)
• Elektrosprej
• (Fenn)
• Desorpce laserem
• za prítomnosti
• matrice (Tanaka,
• Karas, Hillenkamp)

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BLOOD ANALYSIS
Mass calculated 616.1767 Mass measured
616.1761 C34H32N4O4Fe
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ORBITRAP Retainment of the rings

1. Frequencies are determined using a Fourier
   Transform
2. For high sensitivity AND resolution needs
   ultra-high vacuum

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SORI of m/z 740.4703
RESULT DIFFERENT AA RESIDUES ARE PRESENT!!!
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4
5
6
7
8
9
-y
L G F P I N F L T L Y V T V Q H K K L R T P L N Y
I L L N L A V A D L F S
b-
26
27
28
29
30
31
32
33
34
35
36
y6
y5
y7
y9
y4
y8
400
1200
800
2400
2000
1600
b34
b35
b31
b32
b36
b33
MH
b30

b28


b29
b27

b26




2800
3200
3600
4000
m/z
16
13
35
36
35
36
33
34
31
32
29
30
27
28
25
26
23
24
3
4
7
- z
L G F P I N F L T L Y V T V Q H K K L R T P L N Y
I L L N L A V A D L F S
26
27
28
29
30
31
32
33
34
35
36
23
24
25
19
20
17
16
15
14
13
12
11
10
9
8
7
6
5
c -
c16
c13
c17
c20z21
z3
z20
c9
c15
c10
c11
c6
c14
c5
c19
c8
c12
z4
c7
z7
z13
400
1200
800
2400
2000
1600
MH
z35
z25
c36
c34
z24
z36
c33
z32c32
z23
c35
z28
c29
z26
c30
c28
z27
z31
z22
c24
c27
c26
z29
c23
c31
z30
2800
3200
3600
4000
m/z
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IRMPD / ECD Carbonic Anhydrase (29 kDa)
Top Down
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MASS MICROSCOPY
MALDI-LIFT-TOFTOF molecular images reconstructed
from the data obtained by scanning two adjacent
rat brain sections chloroform treated R.
Lemaire et al., Anal. Chem. 2006, eprint ahead
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J. Mass Spectrom. 2006 41 705716
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TRENDY HMOTNOSTNÍ SPEKTROMETRIE - 2006

• Miniaturizace
• Príprava vzorku eliminace práce (DESI a
  jet-assisted DESI)
• Nové analyzátory (orbitrap) / iontová optika
  (zvýšení citlivosti a presnosti)
• Mass microscopy tissue imaging
• Vysoce-rozlišující metody elementární složení a
  moderní disociacní techniky