331 Exonuclease Activity PPTs View free & download

DNA replication PowerPoint PPT Presentation

DNA replication - DNA replication, meselson and stahl experiment, Bacterial- theta replication, viral- rolling circle replication, Eukaryotic replication, telomere replication. 3'-5' and 5' to 3' exonuclease activity

DNA replication, meselson and stahl experiment, Bacterial- theta replication, viral- rolling circle replication, Eukaryotic replication, telomere replication. 3'-5' and 5' to 3' exonuclease activity

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DNA Replication - The experiment by Meselson and Stahl (1957) 2003 spring term. 15. 1 band. H-H ... 3' to 5' exonuclease activity but no nick. Lack 5' to 3' exonuclease activity ...

The experiment by Meselson and Stahl (1957) 2003 spring term. 15. 1 band. H-H ... 3' to 5' exonuclease activity but no nick. Lack 5' to 3' exonuclease activity ...

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DNA Replicates by a Semiconservative Mechanism PowerPoint PPT Presentation

DNA Replicates by a Semiconservative Mechanism - Mechanics of DNA Replication in E. coli. The 5' to 3' exonuclease activity ... Bidirectional Replication of SV40 DNA from a Single Origin ...

Mechanics of DNA Replication in E. coli. The 5' to 3' exonuclease activity ... Bidirectional Replication of SV40 DNA from a Single Origin ...

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Biochemistry 441 Lecture 11 Ted Young March 3, 2000 PowerPoint PPT Presentation

Biochemistry 441 Lecture 11 Ted Young March 3, 2000 - ... conservative replication: the Meselson-Stahl density transfer experiment. ... 5'-3' exonuclease 'nick-translating activity-some bacterial DNA polymerases. 18 ...

... conservative replication: the Meselson-Stahl density transfer experiment. ... 5'-3' exonuclease 'nick-translating activity-some bacterial DNA polymerases. 18 ...

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DNA Replication and Repair PowerPoint PPT Presentation

DNA Replication and Repair - DNA polymerase has 3' to 5' exonuclease function (epsilon-subunit) that ... Terminator utilization substance (Tus) binds to ter region. ...

DNA polymerase has 3' to 5' exonuclease function (epsilon-subunit) that ... Terminator utilization substance (Tus) binds to ter region. ...

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Gene Mutation PowerPoint PPT Presentation

Gene Mutation - How are mutations detected in humans? *Pedigree analysis *Cells cultured in vitro. Analysis of enzyme activity, sequencing of proteins and DNA ...

How are mutations detected in humans? *Pedigree analysis *Cells cultured in vitro. Analysis of enzyme activity, sequencing of proteins and DNA ...

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Genetic Engineering PowerPoint PPT Presentation

Genetic Engineering - Cloning vectors. Simple cloning exercise. Nucleases. exonucleases ... sequences specified by six nucleotides) are good for day-to-day cloning ...

Cloning vectors. Simple cloning exercise. Nucleases. exonucleases ... sequences specified by six nucleotides) are good for day-to-day cloning ...

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Objectives of DNA recombination PowerPoint PPT Presentation

Objectives of DNA recombination - Contain nuclease (exonuclease and Chi-specific ... recR, recQ, recJ ... RecA forms filaments with single-stranded DNA and catalyzes the assimilation of ...

Contain nuclease (exonuclease and Chi-specific ... recR, recQ, recJ ... RecA forms filaments with single-stranded DNA and catalyzes the assimilation of ...

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Organization and Expression of Immunoglobulin Genes PowerPoint PPT Presentation

Organization and Expression of Immunoglobulin Genes - ... therapy resistant dermatitis from 15 days of age, with a diffuse scaling and ... Partial or complete loss of activity (in either RAG-1 or -2) ...

... therapy resistant dermatitis from 15 days of age, with a diffuse scaling and ... Partial or complete loss of activity (in either RAG-1 or -2) ...

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Rules for DNA replication PowerPoint PPT Presentation

Rules for DNA replication - Rules for DNA replication ... DNA polymerases are enzymes that are involved in the template ... DNA polymerase I was the first enzyme discovered with this activity. ...

Rules for DNA replication ... DNA polymerases are enzymes that are involved in the template ... DNA polymerase I was the first enzyme discovered with this activity. ...

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Quantitative RealTime PCR PowerPoint PPT Presentation

Quantitative RealTime PCR - Probe molecules are cleaved during the amplification process to physically ... activity of the polymerase cleaves the probe, releasing the fluorophore ...

Probe molecules are cleaved during the amplification process to physically ... activity of the polymerase cleaves the probe, releasing the fluorophore ...

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Key points for Chapter 6 PowerPoint PPT Presentation

Key points for Chapter 6 - What are the structural differences between DNA and RNA? ... proofreading exonuclease, replisome, pre-replicative complexes (pre-RCs) ...

What are the structural differences between DNA and RNA? ... proofreading exonuclease, replisome, pre-replicative complexes (pre-RCs) ...

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2' DNA Replication, Mutation, Repair PowerPoint PPT Presentation

2' DNA Replication, Mutation, Repair - Repair exonuclease: 5' to 3' yes no no. DNA polymerase III is the main replicating enzyme ... primers on the lagging strand, and it is also a repair enzyme ...

Repair exonuclease: 5' to 3' yes no no. DNA polymerase III is the main replicating enzyme ... primers on the lagging strand, and it is also a repair enzyme ...

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Population Genetics PowerPoint PPT Presentation

Population Genetics - 4 3 2 1 1 = initiator proteins 2 = single strand binding proteins 3 = helicase 4 = topoisomerase (gyrase) Review: Proteins and their function in the early stages of ...

4 3 2 1 1 = initiator proteins 2 = single strand binding proteins 3 = helicase 4 = topoisomerase (gyrase) Review: Proteins and their function in the early stages of ...

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Nomenclature for Triple Test Crosses PowerPoint PPT Presentation

Nomenclature for Triple Test Crosses - Chemistry of DNA - phosphates, deoxyribose, and nitrogen bases (A, G, C, and T) ... Helical molecule with 3.4 and 34 Angstrom Periodicities (Wilkins and Franklin) ...

Chemistry of DNA - phosphates, deoxyribose, and nitrogen bases (A, G, C, and T) ... Helical molecule with 3.4 and 34 Angstrom Periodicities (Wilkins and Franklin) ...

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Exploring Comprehensive Gene Expression Analysis of Prostate Cancer Reveals Distinct Transcriptional Programs Associated With Metastatic Disease Through GenMAPP - Exploring Comprehensive Gene Expression Analysis of Prostate Cancer Reveals Distinct Transcriptional Programs Associated With Metastatic Disease Through GenMAPP ...

Exploring Comprehensive Gene Expression Analysis of Prostate Cancer Reveals Distinct Transcriptional Programs Associated With Metastatic Disease Through GenMAPP ...

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Molecular Biology applications of DNA dependant DNA polymerases PowerPoint PPT Presentation

Molecular Biology applications of DNA dependant DNA polymerases - The holoenzyme DNA polymerase I is no longer as frequently used. ... Synthesis of double-stranded DNA from single-stranded templates ...

The holoenzyme DNA polymerase I is no longer as frequently used. ... Synthesis of double-stranded DNA from single-stranded templates ...

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ENVR 740CHEMICAL CARCINOGENESIS PowerPoint PPT Presentation

ENVR 740CHEMICAL CARCINOGENESIS - ENVR 740 CHEMICAL CARCINOGENESIS Instructor: Avram Gold Office: McGavran-Greenberg 4114C Office phone: 6 7304 Lab: McGavran-Greenberg 3221E Lab phone: 6 7325

ENVR 740 CHEMICAL CARCINOGENESIS Instructor: Avram Gold Office: McGavran-Greenberg 4114C Office phone: 6 7304 Lab: McGavran-Greenberg 3221E Lab phone: 6 7325

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ENZYMES THAT MODIFY DNA AND RNA PowerPoint PPT Presentation

ENZYMES THAT MODIFY DNA AND RNA - enzymes that modify dna and rna 1. restriction endonucleases and methylases restriction endonucleases exist in nature in prokaryotes prokaryotic cells have ...

enzymes that modify dna and rna 1. restriction endonucleases and methylases restriction endonucleases exist in nature in prokaryotes prokaryotic cells have ...

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DNA Replication and Repair - DNA Replication and Repair Replication is bidirectional E. coli genome size = 4.6 X 106 bp Bacteria have circular chromosome with single origin of replication.

DNA Replication and Repair Replication is bidirectional E. coli genome size = 4.6 X 106 bp Bacteria have circular chromosome with single origin of replication.

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DNA Replication The reason why most of us don t have three PowerPoint PPT Presentation

DNA Replication The reason why most of us don t have three - DNA Replication The reason why most of us don t have three eyes. The Three Requirements of Genetic Material Must be able to replicate. Must control the expression ...

DNA Replication The reason why most of us don t have three eyes. The Three Requirements of Genetic Material Must be able to replicate. Must control the expression ...

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DNA Replication - Conservative replication an entirely new double stranded DNA molecule is produced ... Proteins Involved in DNA Replication. Presynthesis. Topoisomerase ...

Conservative replication an entirely new double stranded DNA molecule is produced ... Proteins Involved in DNA Replication. Presynthesis. Topoisomerase ...

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Telomerase - Telomere Terminal Transferase and its Role in Cancer Brian R. Keppler February 27, 2003 The Telomere Found on the ends of eukaryotic chromosomes.

Telomere Terminal Transferase and its Role in Cancer Brian R. Keppler February 27, 2003 The Telomere Found on the ends of eukaryotic chromosomes.

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P1254413619RuKbk PowerPoint PPT Presentation

P1254413619RuKbk - Which of the following statements is true? DNA replication is always bidirectional. DNA replication ... Enzymology of DNA replication. DNA strand separation ...

Which of the following statements is true? DNA replication is always bidirectional. DNA replication ... Enzymology of DNA replication. DNA strand separation ...

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DNA Synthesis PowerPoint PPT Presentation

DNA Synthesis - DNA SYNTHESIS Lecture 31 Mukund Modak, Ph.D.

DNA SYNTHESIS Lecture 31 Mukund Modak, Ph.D.

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Isolation of DNA polymerase I PowerPoint PPT Presentation

Isolation of DNA polymerase I - Isolation of DNA polymerase I. A. Kornberg ... Reaction catalyzed by DNA Pol I. Add dNMP (from dNTP) to 3' end of a growing chain ... Synergism among subunits ...

Isolation of DNA polymerase I. A. Kornberg ... Reaction catalyzed by DNA Pol I. Add dNMP (from dNTP) to 3' end of a growing chain ... Synergism among subunits ...

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RNA Degradation by the Exosome Is Promoted by a Nuclear Polyadenylation Complex PowerPoint PPT Presentation

RNA Degradation by the Exosome Is Promoted by a Nuclear Polyadenylation Complex - RNA Degradation by the Exosome Is Promoted by a Nuclear Polyadenylation Complex ... Physiological condition (150mM salt) 500 mM salt ...

RNA Degradation by the Exosome Is Promoted by a Nuclear Polyadenylation Complex ... Physiological condition (150mM salt) 500 mM salt ...

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DNA Replication I: Basic Mechanism and Enzymology PowerPoint PPT Presentation

DNA Replication I: Basic Mechanism and Enzymology - Mix DNA containing nomal isotope of nitrogen(14N) and DNA labeled with a heavy ... Autoradiograph of replicating embryonic Triturus vulgaris DNA ...

Mix DNA containing nomal isotope of nitrogen(14N) and DNA labeled with a heavy ... Autoradiograph of replicating embryonic Triturus vulgaris DNA ...

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Nucleic acid chemistry part 1 : Some aspects of chemical and enzymatical modification of nucleic acids PowerPoint PPT Presentation

Nucleic acid chemistry part 1 : Some aspects of chemical and enzymatical modification of nucleic acids - Nucleic acid chemistry part 1 : Some aspects of chemical and enzymatical modification of nucleic acids * * * * * * * * Reaction of guanosine with glyoxal (r = ribose ...

Nucleic acid chemistry part 1 : Some aspects of chemical and enzymatical modification of nucleic acids * * * * * * * * Reaction of guanosine with glyoxal (r = ribose ...

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Amplification and cloning of infectious bursal disease virus genomic RNA segments by long and accurate PCR PowerPoint PPT Presentation

Amplification and cloning of infectious bursal disease virus genomic RNA segments by long and accurate PCR - Amplification and cloning of infectious bursal disease virus genomic RNA segments by long and accurate PCR Aydemir Akin, Ching Ching Wu*, Tsang long Lin

Amplification and cloning of infectious bursal disease virus genomic RNA segments by long and accurate PCR Aydemir Akin, Ching Ching Wu*, Tsang long Lin

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DNA sequencing: methods PowerPoint PPT Presentation

DNA sequencing: methods - DNA sequencing: methods I. Brief history of sequencing II. Sanger dideoxy method for sequencing III. Sequencing large pieces of DNA VI. The $1,000 dollar genome

DNA sequencing: methods I. Brief history of sequencing II. Sanger dideoxy method for sequencing III. Sequencing large pieces of DNA VI. The $1,000 dollar genome

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DNA and RNA isolation and purification (course readings 10 and 11) PowerPoint PPT Presentation

DNA and RNA isolation and purification (course readings 10 and 11) - DNA and RNA isolation and purification (course readings 10 and 11) Genomic DNA preparation overview Plasmid DNA preparation DNA purification Phenol extraction

DNA and RNA isolation and purification (course readings 10 and 11) Genomic DNA preparation overview Plasmid DNA preparation DNA purification Phenol extraction

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DNA Repair - Biochemistry

Biochemistry

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The Process of Translation: Elongation and Termination PowerPoint PPT Presentation

The Process of Translation: Elongation and Termination - 4. Rescuing translation when things don't work. binding of aminoacylated tRNA to the A site ... evolved the tmRNA to rescue stalled ribosomes and target the ...

4. Rescuing translation when things don't work. binding of aminoacylated tRNA to the A site ... evolved the tmRNA to rescue stalled ribosomes and target the ...

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DNA repair - Topoisomerase: relieves supercoiling nicks and reaneals one strand of DNA. DNA B: a helicase that separates ds DNA into ss DNA via ATP hydrolysis

Topoisomerase: relieves supercoiling nicks and reaneals one strand of DNA. DNA B: a helicase that separates ds DNA into ss DNA via ATP hydrolysis

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mRNA decay - regulating gene expression - PowerPoint PPT Presentation

mRNA decay - regulating gene expression - - mRNA decay - regulating gene expression - Wiebke Ginter 06.12.10

mRNA decay - regulating gene expression - Wiebke Ginter 06.12.10

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Eukaryotic chromosomes PowerPoint PPT Presentation

Eukaryotic chromosomes - Eukaryotic chromosomes. DNA structure review. Complementary antiparallel strands ... Semiconservative. Parent strand = dark blue. Daughter strand = light blue ...

Eukaryotic chromosomes. DNA structure review. Complementary antiparallel strands ... Semiconservative. Parent strand = dark blue. Daughter strand = light blue ...

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Enzymes for manipulating DNA PowerPoint PPT Presentation

Enzymes for manipulating DNA - Enzymes for manipulating DNA *** Buffers and solution conditions*** I. DNA polymerases III. Kinase and alkaline phosphatase IV. Nucleases V. Topoisomerase

Enzymes for manipulating DNA *** Buffers and solution conditions*** I. DNA polymerases III. Kinase and alkaline phosphatase IV. Nucleases V. Topoisomerase

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DNA Replication and Repair - DNA Replication and Repair. Watson and Crick Predicted Semi-conservative Replication of DNA ... DNA polymerase III major polymerase involved in DNA replication. ...

DNA Replication and Repair. Watson and Crick Predicted Semi-conservative Replication of DNA ... DNA polymerase III major polymerase involved in DNA replication. ...

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Mismatch Repair PowerPoint PPT Presentation

Mismatch Repair - Role of MMR in meiosis. MMR and oxidative DNA damage/alkylation damage and drug tolerance ... AND MSH5 involved in meiosis (crossing over. during meiotic ...

Role of MMR in meiosis. MMR and oxidative DNA damage/alkylation damage and drug tolerance ... AND MSH5 involved in meiosis (crossing over. during meiotic ...

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DNA Replication in Prokaryotes and Eukaryotes PowerPoint PPT Presentation

DNA Replication in Prokaryotes and Eukaryotes - DNA Replication in Prokaryotes and Eukaryotes. Overall mechanism ... Is DNA replication bidirectional? Does DNA replication start at the same location or ...

DNA Replication in Prokaryotes and Eukaryotes. Overall mechanism ... Is DNA replication bidirectional? Does DNA replication start at the same location or ...

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Chapt 21 DNA Replication II: Mechanisms; telomerase PowerPoint PPT Presentation

Chapt 21 DNA Replication II: Mechanisms; telomerase - Chapt 21 DNA Replication II: Mechanisms; telomerase Student learning outcomes: Describe how replication initiates proteins binding specific DNA sequences

Chapt 21 DNA Replication II: Mechanisms; telomerase Student learning outcomes: Describe how replication initiates proteins binding specific DNA sequences

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DNA Replication-III PowerPoint PPT Presentation

DNA Replication-III - DNA Replication-III * * The origin of replication in E. coli is termed oriC origin of Chromosomal replication Important DNA sequences in oriC AT-rich region DnaA ...

DNA Replication-III * * The origin of replication in E. coli is termed oriC origin of Chromosomal replication Important DNA sequences in oriC AT-rich region DnaA ...

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MB 206 : Module 2-C PowerPoint PPT Presentation

MB 206 : Module 2-C - MB 206 : Module 2-C Enzymes used in Gene Manipulation Prepared by Angelia Teo 09

MB 206 : Module 2-C Enzymes used in Gene Manipulation Prepared by Angelia Teo 09

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DNA replication - DNA replication Semi-conservative mechanism 1958, Meselson & Stahl 15N labeling experiment The substrates of DNA synthesis dNTPs dATP, dGTP, dCTP, dTTP Direction ...

DNA replication Semi-conservative mechanism 1958, Meselson & Stahl 15N labeling experiment The substrates of DNA synthesis dNTPs dATP, dGTP, dCTP, dTTP Direction ...

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Objectives of DNA recombination - Homologous recombination of E. coli Identification of genes involved in ... isolation of mutants affecting recombination in recB recC sbcB or recB ...

Homologous recombination of E. coli Identification of genes involved in ... isolation of mutants affecting recombination in recB recC sbcB or recB ...

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To perform fluorescence measurements - To perform fluorescence measurements

To perform fluorescence measurements

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Chapt 20 DNA Replication I: Basic Mechanism and Enyzmology PowerPoint PPT Presentation

Chapt 20 DNA Replication I: Basic Mechanism and Enyzmology - Chapt 20 DNA Replication I: Basic Mechanism and Enyzmology Student learning outcomes: Describe general features of semi-conservative DNA replication: leading, lagging ...

Chapt 20 DNA Replication I: Basic Mechanism and Enyzmology Student learning outcomes: Describe general features of semi-conservative DNA replication: leading, lagging ...

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Welcome Each of You to My Molecular Biology Class PowerPoint PPT Presentation

Welcome Each of You to My Molecular Biology Class - Topoisomerase removes supercoils produced by DNA unwinding at the replication fork ... at which DNA unwinding and initiation of ... Binding and unwinding ...

Topoisomerase removes supercoils produced by DNA unwinding at the replication fork ... at which DNA unwinding and initiation of ... Binding and unwinding ...

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To perform fluorescence measurements - To perform fluorescence measurements

To perform fluorescence measurements

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Primers and Polymerase Chain Reaction PCR PowerPoint PPT Presentation

Primers and Polymerase Chain Reaction PCR - What is PCR and why is ... Benefit: Results in several copies of the target sequence in ... www.wikepedia.com. References. http://www.dnalc.org/ddnalc ...

What is PCR and why is ... Benefit: Results in several copies of the target sequence in ... www.wikepedia.com. References. http://www.dnalc.org/ddnalc ...

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DNA damage and repair summary PowerPoint PPT Presentation

DNA damage and repair summary - HNPCC results from mutations in genes involved in DNA mismatch repair, ... The other strand of the duplex is displaced. Double-strand break repair ...

HNPCC results from mutations in genes involved in DNA mismatch repair, ... The other strand of the duplex is displaced. Double-strand break repair ...

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REPLICATION - GENETICS

GENETICS

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DNA and RNA - DNA and RNA. Four major characteristics for a molecule to serve as ... DNA sequences serve as a repository of all. hereditary characteristics. ...

DNA and RNA. Four major characteristics for a molecule to serve as ... DNA sequences serve as a repository of all. hereditary characteristics. ...

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Section E - DNA Replication PowerPoint PPT Presentation

Section E - DNA Replication - Section E - DNA Replication Contents E1 DNA Replication: an overview Semi-conservative mechanism, Replicons, Origins and termini, Semi-discontinuous replication, RNA ...

Section E - DNA Replication Contents E1 DNA Replication: an overview Semi-conservative mechanism, Replicons, Origins and termini, Semi-discontinuous replication, RNA ...

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Replication: Synthesis of DNA PowerPoint PPT Presentation

Replication: Synthesis of DNA - Relieves torsional strain generated by DNA unwinding. DNA gyrase (DNA topoisomerase II) ... the leading strand proceeds as DNA is unwound by the DnaB helicase. ...

Relieves torsional strain generated by DNA unwinding. DNA gyrase (DNA topoisomerase II) ... the leading strand proceeds as DNA is unwound by the DnaB helicase. ...

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Exonuclease Activity PowerPoint PPT Presentations