Nomenclature for Triple Test Crosses

1
Nomenclature for Triple Test Crosses
2
Properties of Genetic Material

• Transmissible
• Determines heritable physical traits
• Controls Cellular Processes
• Provides source of new variation

3
Elucidation of the Double Helix - Key Evidence

• Chemistry of DNA - phosphates, deoxyribose, and
  nitrogen bases (A, G, C, and T)
• Chargaffs Rule - AT GC
• X-ray crystallography - Helical molecule with 3.4
  and 34 Angstrom Periodicities (Wilkins and
  Franklin)

4
Properties of Genetic Material

• Transmissible
• Use of each strand as template for synthesis of
  complement (semiconservative replication)
• Determines heritable physical traits
• Controls Cellular Processes
• DNA base sequence determines protein sequence
• Provides source of new variation
• Random changes in bases (mutation)

5
Properties of DNA Polymerases I and III

• Template and Primer-dependent 5-3 DNA
  polymerase
• NO 3-5 polymerase activity
• 3-5 exonuclease activity (proofreading)
• 5-3 exonuclease activity (DNA polymerase I only
  - removal of RNA primers on discontinuous strand)