Chapter 15. Gene cloning and DNA analysis in agriculture

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• Chapter 15. Gene cloning and DNA analysis in
  agriculture

???? ?? - ??? ?? - ??? ?? ??? -
Pyrethroids - Organophosphates - ???? ??? - ???
????
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Bacillus thuringiensis? ?-endotoxins - 1904? -
Organophosphates? ?? 8?? ??? ??? - ??? ???
protoxin - ????? ??? ?? ????? ??? ????
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?????? ?-endotoxins cloning - Ostrinia
nubilialis , ?? - ?-endotoxins transgenic maize -
CryIA(b) type - 250 1750 ng? ??? -
Positional effect
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Positional effect
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Gene subtraction - Antisense technology -
Antisense RNA
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Antisense technology - Cellular
ribonuclease - Prevent ribosome from attaching
to the sense strand
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Polygalacturonase polygalacturonic acid
component break-down
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Obtaining transformed plants by A. tumefaciences
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Ploblems with genetically modified plants
Selectable marker? ?? ??? - ??? ?????? ??
kanamycin ???? ??? ?? ????? ???? ???? ?? ????
??? ? ? ??? ?? - ???? ??? ??? ? ? ??? ?? ???
?? ?? ???
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DNA excision by the Cre recombinase enzyme -
Bacteriophage P1 enzyme Cre, 34bp recognition
sequences
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Chapter 16. Gene cloning and DNA analysis in
forensic science
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Genetic fingerprinting - Hypervariable
disperse repetitive sequence - 1980?? - ?? ??
DNA? ?? - ?? ???? ????? ???? ??
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DNA profiling - DNA ?????? STR? ?? ??? ??
??? ?? - ??? DNA - ?? ??? ??
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DNA profiling - ???? ?? kinship analysis -
9?? STR (short tandem repeats) ??
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STR analysis of the Romanov bones -
Mitochondria DNA - ??? - ?? ?? ??
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DNA ??? ?? ?? ?? - ?? Y ??? - ??? , ?? ?? -
??? ?, ???? - ????? ?? ??
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PCR of the amelogenin gene - ?? ?? - ??
????? ???? ??? - Indels ? ?? ? ? ??
Indel ?? ??? ????? ??? ??