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RNA?????

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Author: user Last modified by: ss Created Date: 1/25/2003 7:56:54 AM Document presentation format: Company: user Other titles: Times New Roman ... – PowerPoint PPT presentation

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Title: RNA?????


1

????
RNA?????
(??)
2
  • ?? (transcription)
  • ????DNA?????RNA????
  • ?????????
  • 1. ????DNA
  • 2. ?????????????
  • 3. ????5?3

3
  • ????????

?? ??
?? DNA?? DNA????
?? dNTP (NA?G?C?T) NTP(NA?G?C?U)
?? ?? ???
? DNA??? RNA???
?? DNA RNA
?? A-T?G-C A-U?T-A?G-C
4
  • ???
  • ????

5
  • ??????

??? (coding strand)
???
??? (template strand)
???
????RNA?DNA??
6
  • ??DNA???????????
  • RNA????,?????????
  • ????????????????
  • ??(sense strand)?Watson?,
    ????????(antisense
  • strand)? Crick??

7
  • ????RNA?????,??T?U
  • ?,?????????

8
  • ?????
  • (asymmetric transcription)
  • ?DNA?????????,????
  • ??,???????
  • ??????????????

9
  • ??RNA???(DDRP)
  • 1. ?????RNA???
  • E. coli?RNA??????????
  • ?????( ?2 ? ?? ?)

10
  • E. coli RNA?????

?? ??? ? ?
? 36512 ?????????
? 150618 ????
?? 155613 ??DNA??
? 70263 ?????
11
  • ???????RNA???,????
  • ????????E.coli???
  • ????? RNA????????
  • ?????????????????? ??????RNA??????????
  • ?,?????????

12
  • 2. ?????RNA???

?? I II III
???? 45S-rRNA hnRNA 5S-rRNA tRNA, snRNA
???????? ??? ??? ????
13
  • RNA????????????
  • ?????????????????
  • mRNA???RNA??????
  • ?????,??????????RNA??????????????

14
  • ???????????
  • ?????????????????
  • ??,?????(operon),??????
  • ???????(upstream)??????
  • RNA???????DNA???????
  • ?(promoter)????????????

15
  • ??????RNA??????

16

17
  • ???????

18
  • ???????
  • -35???????TTGACA
  • ?RNA-pol?????
  • -10???????TATAAT
  • ??Pribnow?
  • ?RNA-pol?????

19
  • ???????

20
  • ??????(cis-acting element)
  • ??????????????
  • ??????DNA???
  • ??????(trans-acting factor)
  • ??????????????
  • ?????????????
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