?RNA???? ?????????2002? ? ? ?? ??? ?? - PowerPoint PPT Presentation

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?RNA???? ?????????2002? ? ? ?? ??? ??

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Title: RNA 2002 Author: luoxia Last modified by: Created Date – PowerPoint PPT presentation

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Title: ?RNA???? ?????????2002? ? ? ?? ??? ??


1
?RNA???? ?????????2002?? ??? ??? ??
2
? ?
  • ???RNA???
  • ???RNA???
  • ??RDE-1/Argonaute?miRNA??????
  • ???RNA???
  • ???RNA???
  • 1. miRNA?????
  • 2. miRNA?????????
  • ???RNA???
  • ???RNA???????
  • ????

3
?.?RNA???
  • 1. stRNA (small temporal RNAs )
    ??22??????RNA?
  • 2. siRNA (small intermediate RNAs )
    ??2125??????RNA?
  • 3. miRNA (microRNAs )

4
  • ?.?RNA???
  • miRNAs???????????????????,??stRNAs???????mi
    RNAs?????????3???????5?,stRNAs????????????????mi
    RNAs,??siRNAs??dsRNA?????,???????????,????????????
    ???

5
  • ?. RDE-1/Argonaute?miRNA??????
  • ????,Argonaute orthologs?let-7 ?lin-4
    ??????????,RDE-1?Arabidopsis Ago1?RNAi????????????
    ??24??? RDE-1/Argonaute??,7??Arabidopsis?,4??Droso
    phila?,??4????????RDE-1/Argonautes?????????miRNAs?
    ??????

6
  • ?.?RNA???
  • ??12? miRNAs ??????????????,??90????
    miRNAs ???90????Caenorhabditis briggsae
    ???????????,????????,???????????????,????
    miRNAs,mir-84,?let-7 ?5nt???,??????let-7,?????????
    ??????????????miRNAs???????

7
  • ?.?RNA???
  • miRNA?????
  • ??Drosophila????miRNAs????????????,?????????
    ???miRNAs????????????????????????????,??miRNAs????
    ????,????????????????????????miRNAs???,???????????
    ?????

8
  • ?.?RNA???
  • miRNA?????????
  • ??miRNA??????,??????????let-7
    orthologs?????,????Drosophila?????????????????????
    ??????????, 7???????? miRNA??????????????,???1kb??
    ????????,????????7?miRNA????????????

9
?.?RNA?????????
  • 1.????mRNAs????????
  • 2.?????????mRNA????
  • 3. miRNA???????

10
  • ????mRNAs???????
  • ???let-7 ?lin-4 ?????????,?????let-7 ?lin-4
    ????????mRNAs???let-7?lin-4 RNAs?,???????????????
    ???????????????????????????????RNA??????Drosophi
    la ?????????????,?? mRNAs3???????????????3??????
    ?????????????????????????dcr-1??,????????????

??
11
  • ?????????mRNA????
  • ??????????????????mRNA????????????????
    ?????3??????????????????????,????miRNA???????????
    ??????????????siRNA??????????mRNA????,????miRNA???
    ????

??
12
  • miRNA???????
  • ??????????miRNA?????????????????????,??
    ????Drosoham miRNA????????????????????RNA????????
    ???????????????????miRNA???.

??
13
  • ?.?RNA???????
  • ??70nt?miRNA?????1-1000Kb???????????????,?????????
    ??????Drosphioe??????????????,??miRNA?????????????
    ??????mRNA???????????????????????,?????????,
    miRNA??????????????mRNA????
  • 22nt?siRNA?mRNA????????????????siRNA???????????.
    miRNA?siRNA????????????. miRNA????????,???????????
    ?????

14
????miRNA?????, ???????100?miRNA???30?let-7
, ???????????????miRNA?,??????miRNA???????RNA?????
??,??miRNA???,????????????miRNA???????RNA???????,?
???????????????????????????miRNA??????????????????
??????miRNA,?????????????????????miRNA??????????mi
RNA??????? ???,?RNA???????????,???????????
?????RNA??????????,?????????????,?????????????????
??,???????????RNA???????????
? ?
15
??
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