Microscope Procedure

1
Microscope Procedure
2
Using the COMPOUND scope

• Make sure the stage is in the lowest position
• Position the slide between the slide clips
• Use the COARSE adjustment knob and the left/right
  and up/down knobs to locate your specimen.

3
Using the COMPOUND scope

• When you find your specimen, use the FINE
  adjustment knob to focus.
• When the specimen is FOCUSED AND CENTERED, rotate
  the nosepiece to the LOW POWER objective.
• Focus using the FINE adjustment.
• NEVER use the COARSE ADJUSTMENT knob to focus on
  LOW or HIGH POWER.

4
Using the COMPOUND scope

• When finished, wrap the cord, replace the dust
  cover, and put the microscope back in the
  cabinet.

5
Using the STEREOSCOPE

• Place your specimen on the stage.
• Turn on the light (either bottom or top)
• Use the knobs to raise and lower the eyepieces to
  focus.

6
Using the STEREOSCOPE

• When finished, wrap the cord, replace the dust
  cover, and put the microscope back in the
  cabinet.
• All of this information is reviewed in the
  Podcast section of the Zoology Website
• http//staff.norman.k12.ok.us/catherines/

7
Estimating Sizes

• Remember from Biology
• The Field of Vision - area you can see when
  looking through the eyepiece.
• Scanner 3750 microns
• Low Power 1500 microns
• High Power 375 micron

8
Estimating Sizes

• Estimate how many of your objects can fit across
  the diameter.
• Divide the diameter (3750 or 1500 or 375) by the
  estimated number of objects.

9
Estimating Sizes
On Low Power 1500 microns 1500/30500 microns