Especially attacked young animals

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COLIBACILLOSIS
Cause E. coli

• Especially attacked young animals
• Human

• Infant Acute gastroenteritis
• Adult Cystitis

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Animal

• Pig
• - Gut Oedema Odema Disease BowI oedema
• - Young pigs cause enteric colibacillosis
• Cow
• - Secunder Infection Omphalitis, Arthritis,
  Cystitis,
• Mastitis, Pyelonephritis
• Avian
• - Hjarres Disease Colligranuloma
• - Omphalitis, Peritonitis, Salphingtis

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ETHIOLOGY
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• Easy grow at every media
• Ex blood agar, Beta haemolitik
• Mac Conkey - wet red coloni
• EMBA - green methalic coloni with black at the
  center
• Fluid media - muddy
• Sensitive with dry conditing and desinfectan
• Death at 600 c for 30 mnt
• Inside ice cube still alive for 6 month

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E. COLI GROOVE CAUSE OF ENTERISTIS
1. E. Coli Entero Toxigenic (ETEC) 2. E. Coli
Entero Pathogenic (EPEC) 3. E. Coli Entero
Invasive (EIEC) 4. E. Coli Adhesive and Emerge
(AEEC)
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EPIZOOTIOLOGI

• In Indonesia several cause
• Less information
• At a common disease for young animals
• Young cow ? 25-30
• Young horse ? 25
• Young pig ? 50

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LOSS FINANCIAL

• Valve of treatment
• Body weight losses

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PATHOGENESIS
MECHANISMS OF INFECTION

• Source of infection
• - Feces
• - Surrounded
• - Salphingitas
• - Matching egg
• Route of infection
• - GIT
• - Milk
• - Intra uterine infection

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Vicious disease depend on

1. Status imunity of the host
2. Bact. ability to produce toxin

• Cow
• a. Septicaemia colibacillosis
• b. Enteric toxaemia colibacillosis
• c. Enteric Colibacillosis

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• Pig cause 3 types of enteric colibacillosis
• a. Neonatal enteritis
• b. Weanling enteritis
• c. Edema Disease (enterotoxaemia)
• Sheep (2 type)
• a. Enteric proliferation groove
• E. Coli ? enterotoxin out come
• b. Bacteremic

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• Horse
• a. Young horse
• Symptom temp, pulse ?, weakness after 24
  hour
• b. ? Horse
• Cause metritis after dystokia

CLINICAL SYMPTOM
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Dog

• Fading puppy syndrom
• Symptom
• weakness, anorexia,
• Dog
• 70 cause of pyometra

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CHICKEN

• Young chicken
• 3 weeks cause of pericarditis
• Chicken
• resp. tract with IB virus and ND

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BACTERY DIFFUSE IN BLOOD
Cause

• a. Acute colisepticaemia
• b. Serositis
• c. Coligranuloma (Hjarres Disease), with
  spesific tag granulomatous at the intestine wall,
  liver and pulmo

Infected
a. Inhalation b. Egg contamination with faeces
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YOUNG COW
3 types of colibacillosis

• a. Enteric toxaemia colibaccillosis, collaps,
  in 2 - 6 hour, diarrheae (-)
• b. Septicaemia colibacillosis, acute, death for
  1-4 days.
• c. Enteric colibacillosis, attacks
• 1-3 weeks, fatty feces, 3-5 days

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YOUNG SHEEP
type

• Septicaemia per acute and enteric chronic
• Sensitive 1-2 days
• Per acute, sudent death
• Chronic, occur arthritis

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YOUNG PIG

• Attack 1 - 3 days ages
• Sudden death for 24 hours without clinical
  symtomp
• Enteric colibacillosis, attacked 8 - 16 weeks
• Clinical symtomp, depresion, anorexia, 40,50C,
  diarrhaea

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PA CHANGES

• Enteric toxaemia and septicaemic
• Colibacillosis non specific
• Gastritis, enteritis
• Avian Pericarditis, peritonitis
• Chicken omphalitis

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DIAGNOSIS

• Material specimen
• Cardiac blood coagulant
• Intestine contents
• Mesenterials
• Lien
• Cerebrospinal fluid
• Faecal swab

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Diagnosis based on
clinical diagnosa

• Difficult because of the manifestation of the
  disease similar with other disease
• Bacteria isolated and identification
• Isolated
• Identification sugar, biochemisty

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DIFFERENTIAL DIAGNOSA

• Salmonellosis
• Diarrheae because of feed
• Acute septic of disease

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DISEASE CONTROL

• Prevention
• - Avoid from the the denity
• - Diarrheae because of feed
• - Acute septic of disease
• Administration
• - The farmer should report rapidly
• - D/by lab
• R/
• - Oxytetracycline
• - Streptomycine
• - Kanamycin

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INTRODUCTION

• Infectious Disease in animals human
• Caused by Salmonella
• Food Borne Disease (eggs, milk and meat products)
• Animals ? Decrease of production
• Enteric Disease
• Abortus and neonatal death
• Fowl ? High Mortality rate (DOC up to 2 3
  weeks of age)

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Etiology

• Salmonella ? road shape cell, Gram negative, non
  spore, somatic (O) flagellar (H) antigens
• Salmonella are usually motile except in poultry
  S. gallinarum S. pullorum, do not ferment
  lactose.
• Growth optimal in MCA, SSA
• The genus Salmonella contains more than 2.400
  serotypes, occur worldwide and infect many
  mammals, birds and reptiles and are mainly
  excreted in faeces.

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Epizootiology Salmonellosis is a worldwide
zoonosis, besides economic losses to animal
production, its zoonotic character makes it an
important public health problem. Organism may be
present in water, soil, animal feeds,raw meat and
in vegetable material. The source of
environtmental contamination is faeces In poultry
? S. pullorum infect the ovaries, and the
organisms can be isolated from eggs.
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Pathogenesis Although many aspects of the
pathogenesis of Salmonellosis are poorly
understood, the virulence of Salmonellae relates
to their ability to invade host cells, replicate
in them and resist both digestion by
phagocytes. Peroral ? tract. Intestinalis
(mucosae of the illeum, caecum and colon) and the
messenteric lymph nodes ? replicate and LPS
product endotoxin ? it may be contribute to the
local inflammatory response which damages
intestinal epithelial cells ? diarrhoea ?
organisms can be excreted through faeces
Bacterial cell wall LPS also mediates the
endotoxic shock ? septicaemic salmonellosis Latent
infections, in which Salmonellae are present in
the gall bladder but are not excreted.
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• Clinical Symptom
• Clinical disease may develop from subclinical and
  latent infections if affected animals are stessed
  e.g transportation, overcrowding etc.
• Salmonellosis in adult cattle is usually sporadic
  and is also often associated with stress.
• In most animals species, both enteric and
  septicaemic forms of salmonellosis are recorded.
• Enteric salmonellosis
• Acute disease is characterized by fever,
  depression, anorexia and profuse diarrhoea often
  containing blood, mucus and epithelial casts.
  Dehydration and weight loss follow and pregnant
  animals may abort.

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• Chronic disease
• Can follow acute Salmonellosis in pigs, cattle
  and horses are characterized by intermittent
  fever, soft faeces and gradual weight loss,
  emaciation.
• Septicaemic Salmonellosis
• Clinical disease is sudden with high fever,
  depression, and die within 48 hours.
• Surviving animals can develop persistent
  diarrhoea, arthritis, meningitis or pneumonia.
• Pigs ? characteristic bluish discolouration of
  the ears and snout.

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Salmonellosis in poultry
S. pullorum ? Pullorum disease S. gallinarum ?
Fowl typhoid S. enteritidis in egg dishes may
result in human food poisoning PULLORUM DISEASE
Bacillary White Diarrhoae ? Infects young chicks
and turkey poults up to 2 3 weeks age and the
mortality rate is high The clinical symptoms are
anorexia, depression, whitish faecal pasting
around their vents and characteristic lessions
include whitish nodes throughout the lungs and
focal necrosis of liver and spleen. FOWL TYPHOID
? Lession in young chicks and poults similar of
Pullorum Disease Characteristic findings
bile-stained liver and enlarge spleen.
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Differentiation of the biotypes of S. pullorum
and S. gallinarum
S. pullorum S. gallinarum
Glukose (gas) - Dulcitol - Maltose -
Ornithine decarboxylase - Rhamnose -
Motility - -
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Diagnosis

• A history of previous outbreaks of the disease,
  clinical sign and postmortem picture.
• Laboratory confirmation is required
• Specimens faeces, blood, intestinal contents,
  tissue lession and abomasal contents ( aborted
  foetuses )
• - Isolation and identification ? specimen should
  be cultured onto SSA, BGA and also added to
  Selenite F, Tetrathionate broth for enrichment.
• - Serrological tests ? Rapid Whole Blood Test
  (RWBT) for Pullorum Test with Polyvalent antigen
  or ELISA and DNA probes.

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Treatment

• Antibiotic therapy should be based on results of
  susceptibility testing
• Oral antimicrobial therapy should be used
  judiciously for treating enteric salmonellosis
• Fluid and electrolyte replacement therapy is
  required to counteract dehydration and shock

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Control

• Measures for excluding infection form a herd or
  flock free of salmonellosis
• Measures for reducing environmental contamination

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Thank You