??????cDNA?????????????????

1
????????????????????????????????????

• ???
• ???????

2
???????

• ????????
• ??????
• ??????????????????????????
• ????????
• ??????cDNA?????????????????
• ??????
• GCG, BLAST, FastA,
• ???????????(?)
• ??????,????
• ??????,???????
• ????

3
?????????

• Bioinformatics
• Infomation biology (Infobiology)
• Computational biology

Biologist
Informaticist
Statisticist
4
??????????

• ?????????????
• ???????????????
• ???????????
• ???????????

5
??????????

• Genome projects
• gt500 viruses, gt25 bacteria, Caenorhabditis
  elegans, Saccharomyces cerevisiae, Drosophila
  melanogaster Human, mouse, rice,
• Post-genomic era
• Genomics, Drug discovery, Disease gene finding,
  Molecular evolution, Biotechnology,
• Positional candidate cloning vs functional cloning

6
??????????

• ??????
• ??????(ISI) 8,657
• hiv (PubMed) 89,174 12 (1984) 8,235 (1999)
  241 (1999, JV)
• ?????
• gt800 organisms
• EST(??????)
• February 19, 1999 1,270,291 (human) 2,153,771
• July 28, 2000 2,208,221 (human) 5,292,022

7
??????????
8,604,000,000 bases in 7,077,000 sequence
records
June, 2000
8
?????????? ?RSDB??

• ?????? (????????)
• ????????
• ???????????
• ???????????????
• ??????
• ?????????
• ???? (?????????????????????)

9
???????

• ???????????
• ????????
• ??????
• ??????????????????????
• ????????????

10
????????????

• ????
• ISIS Draw, Array Viewer, Spotfinder, Lucy,
  ClustalW, Glimmer, Staden (Unix), AAT, MUMmer,
  Annotator, Assembler, btab, Blast2,
• ????
• VectorNTI, MacVector, DNAsis, Plasmid Premier,
  Primer Premier 5, Array Disigner, Spotfire,

11
????????

• ???
• PDB, Genbank, EMBL, SwissProt, dbEST, HTGs,
  dbSTS, TransFac, PubMed, OMIM, TDB, KEGG, USPTO,
  
• ?????????
• ExPASy, Staden, GCG, BCM launcher, Biology
  workbench, Deambulum,
• ????,?????????
• POST,
• ????
• WebBio, Biohunt, Nee-How, InFoseek, Yahoo, Alta
  Vista, GAIS,

12
???????? ?????????

• Sanger Ceter, Genome Research (DOE), Joint Genome
  Institute (DOE), Human genome center (LLNL), hgsc
  (BCM), TIGR, Human Genome Projecy (ELSI), Genome
  Sequencing Ceter (wustl), NHGRI,
• Entrez Genome View (NCBI), The Human Transcript
  Map (NCBI), Genome Catalog (ORNL),
• Human Genome Advanced Annotation Tutorial,

13
???????? ??????

• NCBI (National Center for Biotechnology
  Information) Entrez (PubMed)
• NHRI (National Health Research Institute) Hint
• ExPASy SeqAnalRef
• Medscape

14
???????? ????????

• NCBI (National Center for Biotechnology
  Information) Entrez
• EBI (European Bioinformatics Institute) SRS
• GenomeNet (Institute for Chemical Research, Kyoto
  University)
• DBGET/LinkDB, STAG
• DDBJ (DNA Data Bank of Japan, National Institute
  of Genetics)
• getentry by accession numbers
• Sfgate WAIS by key words
• SRS by key words

15
???????? ??????

• ????
• BCM launcher
• ExPASy
• DEAMBULUM
• ????
• GCG
• Staden
• Biology workbench

16
???????? ??????

• Apipa
• ????????????????
• ?????????DNA?????
• ??????
• IPN, USPTO, EPO
• ?????????
• ?????????DNA?????
• CA-STN, Beilstein
• ?????,???????
• ??????????

17
???????????

• ?????????
• POST
• ????????
• WebBio, Nee-How
• ???
• SRS, RSDB, PDB, SpecInfo, CSD, ICSD, ISIS, TWP,
  ????????
• ?????????
• GCG, Staden, ExPASy
• ??
• Hint, STICNET