Suicide Ideation in the depression group (NBB)

1
Suicide Ideation in the depression group (NBB)

• A medical term for thoughts about or an unusual
  preoccupation with suicide. The range varies
  greatly from fleeting to detailed planning, role
  playing, self-harm and attempts

Anterior Cingulate Cortex



DLPFC was also checked, however, none of the
target genes were unchanged.
Juan Zhao and Dick Swaab, submitted
2
(No Transcript)
3
(No Transcript)
4
Microglia isolation procedure

• Mechanic enzymatic dissociation
• Cell separation Percoll gradient
• Take off myelin
• Collect cells from interface
• Stain cells with labeled antibodies
• CD11b - PE
• CD45 - FITC
• CD15 - APC
• Selection using magnetic CD11b beads

Melief et al. Glia 2012
5
Isolation and phenotyping of pure primary human
microglia
6
Peripheral inflammation impacts on microglia
activation
Melief et al. Glia 2012
7
(No Transcript)
8
The Netherlands Brain Bank for Psychiatry
9
Phenotype Characterize human post mortem
microglia
Jeroen Melief, thesis 2014
10
(No Transcript)