Diapositive 1

1
Supplemental Fig. 2. Pattern of expression of
DsRed and GFP for the 12 monoclonal populations
selected from stable transfections. HeLa cells
were transfected with the Not1-linearized
reporter plasmids as shown in Fig. 1A, antibiotic
resistant cells were selected and monoclonal
populations were generated by single cell
sorting. Clones were characterized as described
in the main text and the GFP and DsRed expression
was analyzed by cytofluorometry. The six panels
on the left depict clones carrying the plasmid
with convergent reporter gene transcription and
the six clones on the right hand side have
divergent reporter genes. (A) Representative
telomeric clones expressing undetectable or low
levels of the reporter proteins. (B)
Representative telomeric clones expressing
detectable levels of the reporter proteins. (C)
Representative clones generated from
transfections with the plasmid devoid of
telomeric repeats, and showing a non-telomeric
location by FISH analysis.