RapidVet

1
RapidVet-HBlood Group Determination Systemfor
Identifying DEA 1.1 Positive and DEA 1.1 Negative
Dogs
2
Kit containsauto-agglutination saline screen
cards, patient test cards, pipettes, stirrers,
diluent, instructions, interpretation guideand
report card.Shelf-life 24 months at room
temperature
3
If agglutination is observed, STOP the test and
perform cell washing procedures before
proceeding.
Prior to typing, check for auto-agglutination
using the card provided in the kit. This card is
used for three patients and, after use, should be
returned to the kit box.
If auto-agglutination does not occur, proceed to
the next step.
4
Remove the Test Card from its plastic sleeve.
Write the name/number of the dog and the testing
date on the card.
5
Dispense 1 drop of diluent from the dropping
bottle into each well.
6
Aspirate a small amount of patient sample into a
pipette and release 1 drop (50µl) into each well,
taking care not to cross-contaminate the wells.
7
Using a NEW stirrer and pressing downward with
the flat portion of the stirrer, spread and mix
the materials within the entirety of the first
well for about 10 seconds.
8
Using a NEW stirrer, spread and mix the materials
within the entirety of the second well for about
10 seconds.
9
Using a NEW stirrer, spread and mix the materials
within the entirety of the third well for about
10 seconds.
10
Rock the card, using a transaxial motion, for 1
minute.
Be sure materials are mixing and rotating
within each individual well. Be careful not to
cross-contaminate.
11
Read the results, noting whether agglutination
has or has not occurred in the Patient Test
well.
DEA 1.1?
DEA 1.1
12
After the card has been read, take a digital
photograph for a permanent record, or allow to
dry and replace in its plastic sleeve as a
permanent record.