Fluorescence

1
Fluorescence
2
Topics

• Sensitivity (nM-pM)
• Contrast UV-vis measurements
• Derivatization
• LIF

3
Atomic Absorption Spectroscopy

• Elemental analysis
• Dissolved metals
• Source
• Hallow Cathode Lamp
• Cathode of specific element
• Sample
• Acetylene-air flame, nebulizer
• Detector
• PMT

4
Atomic absorption

• Gas-phase elemental atoms
• Narrow absorption lines (0.01 nm)
• Element specific source
• Flame conditions control sensitivity
• 1-100 ppm (mg/L)
• varies with element

5
AA Spectrometer
PMT
Hollow cathode lamp
Flame
monochrometer
Coating of element
Air
nebulizer
Acetylene
sample
6
Experiment

• Aspirate blank, set 100 T
• Aspirate sample
• Matrix effects are common
• Standard addition analysis

7
Atomic Emission Spectroscopy