Title: Microbial Genetics
1Microbial Genetics
- Mutation
- Genetic Recombination
- Model organism
- E. coli
- Molecular Cloning
- Bioinformatics
Golden Rice
2Plasmid Types
- Resistance Plasmids R Plasmids
- Virulence Plasmids
- Bacteriocins
- Engineered Plasmids
3Mutants
- Mutation
- Wild Type
- Genotype
- Phenotype
4Isolation of Mutants
- Replica Plating
- Auxotrophs Nutritional Mutants
- Prototroph
- Wild Type
5 Penicillin Selection Method Kills Growing
Cells
6Mutations
- Spontaneous Mutations
- Point Mutations
- Base-Pair Substitution
- Silent,
- Missense, or
- Nonsense Mutations
7Conditional Lethal Mutations
- Temperature Sensitive Mutations
Incubation Temperature
30 C
40 C
8Reading Frame Frame Shift Mutation
9Reversion
Mutation Prototroph Auxotroph Back
Mutation
- Back
- Mutation
- Suppressor
- Mutation
Mutation Back Mutation
Normal Protein
10Other Mutations
- Deletions Lose of a Piece of DNA
- Insertions Additions of a Piece of DNA
- Translocation Movement of a Piece of DNA to a
New Location in the Genome
11Inversion
- Orientation of a Piece of DNA is Reversed in the
Genome - Phase Variation
12Mutation Rate
- Spontaneous (Natural or Background) Mutation Rate
- DNA 1 per Million (varies between genes and
location) - RNA 1 per Thousand
- Mutagens Anything that Increases the Mutation
Rate
13Mutagens
- Chemicals
- Base Analogs Chemically Resemble Base
14Intercalating Agent Inserts Between Base Pairs
15- Radiation
- UV Light
- Ionizing Radiation
- DNA Repair
- SOS Regulatory System
16Biological Mutagens
- Transposons Can Insert Into Gene
- Transposon Mutagenesis
- Site Directed Mutations Man-made
17Ames Test
- Mutagens ? Carcinogens
- Ames Test Measures Back Mutation Rates to Detect
Carcinogens - Liver extract
18Bacterial Gene Transfer
- Genetic Recombination
- 3 Types of Bacterial Gene Exchange
- 1. Transformation
- Naked DNA
- Competent
19- Transfection in Eukaryotes
- Particle (Gene) Guns Insert DNA
- Electroporation
202. Transduction
21 Specialized Transduction
22 Lysogeny Can Lead to Phage Conversion
233. Conjugation
- Plasmids
- Copy Number
- Incompatibility
- Curing
- Conjugative Plasmid
24Chromosomal Mobilization
- Fertility (F) Plasmid
- Episome
- HFr Strain
- Sex Pili
25Gene Maps
26E. coli Chromosome
- 4,639,221 Base Pairs
- 4,288 Open Reading Frames
- 88 ORF
- 1 rRNA, tRNA
- 10 Promoter,
- Origin, etc
- 6 Operons
27Lateral Gene Transfer
- Horizontal Gene transfer
- Pathogenicity Islands
- ICE Integrative and Conjugative Elements
28Transposition
- Transposase
- Conservative Transposition
- Replicative Transposition
- Phase Variation
- Inversion
29Genomics
- Mycoplasma genitalium Genome
- 470 Open Reading Frame
- Genomic Mining
30(No Transcript)
31Genetic Word List
Genotype Phenotypes Wild-Type Isolation
Mutants Replica plating Auxotroph
Prototroph Penicillin-Selection Method
Spontaneous Mutations Point Mutation Base-Pair
Substitution Silent, Missense, Nonsense Mutations
Mutation Genetic Recombination Microbial
Models Molecular Cloning Biotechnology Mutants
32Genetic Word List 2
Temperature Sensitive Mutation Reading
Frame Frame Shift Mutation Back
Mutations Suppressor Mutation
Deletions Insertions Translocation Inversion Mutat
ion Rate DNA 10-6 RNA 10-3
Mutagens Chemicals Base Analogs Intercalating
Agents Radiation UV Light
33Genetic Word List 3
Ionizing Radiation DNA Repair SOS Regulatory
System Biological Mutagens Transposons
Site Directed Mutations Carcinogenesis Ames
Test Genetic Recombination
Bacterial Gene Transfer Transformation Competent T
ransfection Electroporation Particle gun
34Genetic Word List 4
Conjugation Plasmids Copy Number Incompatibility C
uring Conjugative Plasmids
Transduction Generalized Transduction Specialized
Transduction Lysogeny Phage conversion
Resistance Plasmids R Plasmids Virulence
Plasmids Bacteriocin Engineered plasmids
35Genetic Word List 5
Chromosomal Mobilization Sex Pili Fertility (F)
Plasmids HFr Strains Interrupted Matings Genetic
Maps
Escherichia coli Chromosome 4,639,221 Base
Pairs 4,288 Open Reading Frames Horizontal Gene
Transfer
Transposition Transposase Conservative
Replicative Transposition Phase
Variation Inversion
36Genetic Word List 6
Pathogenicity Islands Genomics Mycoplasma
genitalium Genome 470 Open Reading
Frames Genomic Mining