Polarization Toolbox

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• Polarization Toolbox
• Electro-Optical L-Format (EOLF) Polarization of
  Fluorescence Module
• Add Anisotropy and Circularly Polarized
  Luminescence Easily

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Traditional L-Format Anisotropy
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Traditional Calculation of Anisotropy and
Polarization
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Anisotropy with New EOLF
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Calculation of Anisotropy and Polarization using
an EOLF Module
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EOLF Advantages

• No Gain Correction Factor (G-Factor)
• No polarizer movement
• Rapid switching between polarization states
• supports high speed kinetic experiments

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EOLF Chamber, PEM version
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EOLF Chamber in a DM 45 Spectrofluorimeter
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Comparison of PEM and LCVR Properties
Property PEM LCVR
Optical Aperture 17 mm 18 mm
Optical Material Supracil Nematic Liquid Crystal
Operating Frequency 50 KHz 10 Hz or Lower
Wavelength Range 170 - 1400 nm 340 to 2000 nm
Waveform of Retardance Changes Sinusoidal Square wave (IR and IL)
Static Retardance No Yes
Adjusting Retardance LCPL RCPL (only one voltage) LCPL and RCPL must be set independently (two voltages)
Kinetic Measurements milliseconds seconds
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Photon Counter Gating
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Excitation Anisotropy Spectrum of Rhodamine
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Fluorescein Spectra as a Function of Temperature
temperature
Increasing

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EOLF Also Supports CPL
Circularly Polarized Luminescence (CPL)

• Differential emission of left and right
  circularly polarized light
• Fluorescence analog of circular dichroism (CD)
• Sensitive to environmental changes of chiral
  fluorophores

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(No Transcript)
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Left and Right Polarized Emission Spectra
Europium(III) tris(trifluoromethylhydroxymethylene
)-d-camphorate in DMSO
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CPL Spectrum of Europium Compound
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CPL with Polarized Excitation
Use circular polarized excitation to photoselect
one enantiomer in a racemic mixture
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US Patent Pending
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EOLF Adds Complementary Techniques to a Single
Optical Bench
Measurement Selectivity Sensitivity
Absorbance Chromophore Ground state environment
Fluorescence Fluorophore Excited state environment
POF (anisotropy) Fluorophore Excited state mobility
CD Chiral chromophore Ground state symmetry
CPL Chiral fluorophore Excited state symmetry
FDCD (fluorescence detected circular dichroism) Chiral fluorophore Ground state symmetry
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With One EOLF, Measure

• Conformation characterization of proteins and
  other biological macromolecules
• Equilibrium binding studies of macromolecules
• Characterization of metal ligand complexes
• Characterization of entantiameric mixtures