Polarization Toolbox. Electro-Optical L-Format (EOLF) ..

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• Polarization Toolbox
• Electro-Optical L-Format (EOLF) Polarization of
  Fluorescence Module
• Add Anisotropy and Circularly Polarized
  Luminescence Easily

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Traditional L-Format Anisotropy
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Traditional Calculation of Anisotropy and
Polarization
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Anisotropy with New EOLF
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Calculation of Anisotropy and Polarization using
an EOLF Module
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EOLF Advantages

• No Gain Correction Factor (G-Factor)
• No polarizer movement
• Rapid switching between polarization states
• supports high speed kinetic experiments

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EOLF Chamber, PEM version
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EOLF Chamber in a DM 45 Spectrofluorimeter
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Comparison of PEM and LCVR Properties
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Photon Counter Gating
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Excitation Anisotropy Spectrum of Rhodamine
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Fluorescein Spectra as a Function of Temperature
temperature
Increasing

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EOLF Also Supports CPL
Circularly Polarized Luminescence (CPL)

• Differential emission of left and right
  circularly polarized light
• Fluorescence analog of circular dichroism (CD)
• Sensitive to environmental changes of chiral
  fluorophores

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Left and Right Polarized Emission Spectra
Europium(III) tris(trifluoromethylhydroxymethylene
)-d-camphorate in DMSO
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CPL Spectrum of Europium Compound
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CPL with Polarized Excitation
Use circular polarized excitation to photoselect
one enantiomer in a racemic mixture
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US Patent Pending
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EOLF Adds Complementary Techniques to a Single
Optical Bench
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With One EOLF, Measure

• Conformation characterization of proteins and
  other biological macromolecules
• Equilibrium binding studies of macromolecules
• Characterization of metal ligand complexes
• Characterization of entantiameric mixtures