Polarization%20Toolbox - PowerPoint PPT Presentation

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Polarization%20Toolbox

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... Ground state symmetry Chiral chromophore CD Excited state ... No excitation polarizer necessary. PEM used in quarter wave application. For isotropic ... – PowerPoint PPT presentation

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Title: Polarization%20Toolbox


1
  • Polarization Toolbox
  • Electro-Optical L-Format (EOLF) Polarization of
    Fluorescence Module
  • Add Anisotropy and Circularly Polarized
    Luminescence Easily

2
Traditional L-Format Anisotropy
3
Traditional Calculation of Anisotropy and
Polarization
4
Anisotropy with New EOLF
5
Calculation of Anisotropy and Polarization using
an EOLF Module
6
EOLF Advantages
  • No Gain Correction Factor (G-Factor)
  • No polarizer movement
  • Rapid switching between polarization states
  • supports high speed kinetic experiments

7
EOLF Chamber, PEM version
8
EOLF Chamber in a DM 45 Spectrofluorimeter
9
Comparison of PEM and LCVR Properties
Property PEM LCVR
Optical Aperture 17 mm 18 mm
Optical Material Supracil Nematic Liquid Crystal
Operating Frequency 50 KHz 10 Hz or Lower
Wavelength Range 170 - 1400 nm 340 to 2000 nm
Waveform of Retardance Changes Sinusoidal Square wave (IR and IL)
Static Retardance No Yes
Adjusting Retardance LCPL RCPL (only one voltage) LCPL and RCPL must be set independently (two voltages)
Kinetic Measurements milliseconds seconds
10
Photon Counter Gating
11
Excitation Anisotropy Spectrum of Rhodamine
12
Fluorescein Spectra as a Function of Temperature
temperature
Increasing

13
EOLF Also Supports CPL
Circularly Polarized Luminescence (CPL)
  • Differential emission of left and right
    circularly polarized light
  • Fluorescence analog of circular dichroism (CD)
  • Sensitive to environmental changes of chiral
    fluorophores

14
(No Transcript)
15
Left and Right Polarized Emission Spectra
Europium(III) tris(trifluoromethylhydroxymethylene
)-d-camphorate in DMSO
16
CPL Spectrum of Europium Compound
17
CPL with Polarized Excitation
Use circular polarized excitation to photoselect
one enantiomer in a racemic mixture
18
US Patent Pending
19
EOLF Adds Complementary Techniques to a Single
Optical Bench
Measurement Selectivity Sensitivity
Absorbance Chromophore Ground state environment
Fluorescence Fluorophore Excited state environment
POF (anisotropy) Fluorophore Excited state mobility
CD Chiral chromophore Ground state symmetry
CPL Chiral fluorophore Excited state symmetry
FDCD (fluorescence detected circular dichroism) Chiral fluorophore Ground state symmetry
20
With One EOLF, Measure
  • Conformation characterization of proteins and
    other biological macromolecules
  • Equilibrium binding studies of macromolecules
  • Characterization of metal ligand complexes
  • Characterization of entantiameric mixtures
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