A1261687514VEHzq

1
Supplemental material, Wen, et al.
Fig. 1 Nucleotide sequences. Detailed nucleotide
sequence of the double-strand DNA with 3
single-strand DNA overhang substrate is shown in
(A). Substrates used in Figure 4D were made by
combination of annealing oligonucleotides,
presented (5 to 3) in (B). The partial fork
substrate having a 25 bp duplex lagging or
leading strand arm were made by annealing
combinations of a, b, c, or d for partial forks,
and b, d or e for linear duplex DNAs.
(A)
(B)
( a ) GTCGGATCCTCTAGACAGCTCCATGATCACTGGCACTGGTA
GAATTCGGC (b) CAACGTCATAGACGATTACATTGCTACATGGAGC
TGTCTAGAGGATCCGA (c) TGCCGAATTCTACCAGTGCCAGTGAT
(d) TAGCAATGTAATCGTCTATGACGTT (e)
GTCGGATCCTCTAGACAGCTCCATG (f) GACGCTGTCGACATCGGTA
GGTTCTTTCCCGCATTCGAGTATCACTGGCACTGGTAGAATTCGGC
Figure 2. Binding of wild-type Mre11 and
?5-7Mre11 bind to fork like substrates containing
different lengths of ssDNA. Percentage of the
added fork-like substrates bound by wild type or
mutant Mre11 in the gel mobility-shift assays
presented in Fig. 3D were determined. The
concentration of proteins is 1?M and
concentration of labelled substrates is 1 nM.