IMViC

1
IMViC

• Indole (3 tests)
• Methyl Red
• Voges-Proskauer
• Citrate
• Experiment 25 A 26

2
Indole

• Objective 1determine ability of your organisms
  to degrade the amino acid tryptophan (an amino
  acid) into metabolic products using tryptophanase
  (enzyme).
• TryptophangtgttryptophanasegtgtIndole Pyruvic acid
  ammonia
• Metabolic (nutritional)Pyruvic Acid Ammonia
• Waste ProductIndole
• Kovacs reagent extracts reacts with indole in
  agar and produces a fushia color on surface

3
Indole

• Objective 2determine your organisms ability to
  hydrolyze sulfur containing amino acids
  (cysteine) or reduce inorganic sodium
  thiosulfate.
• Two Pathways
• Organic
• cysteingtgtcysteine desulfurasegtgtPyruvic acid
  hydrogen sulfide ammonia
• Inorganic
• Thiosulfategtgtthiosulfate reductasegtgtsulfite
  hydrogen sulfide
• Hydrogen sulfide reacts with ferrous ammonium
  sulfate to produce a black precipitate

4
Indole

• Inoculate SIM tube with needle
• Incubate 37C Read Thursday
• Black precipitant () hydrogen sulfide
• Add Kovacs reagent
• Fushia on top () indole
• Check motility

5
Indole

• Objective 3determine if your organism has
  flagella (can confirm motility test)
• SIM agar Soft agar that contains tryptophan,
  peptones that are the only carbon source
  (cysteine), ferrous ammonium sulfate and sodium
  thiosulfate.

6
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