????????

1
??? ???????????????

• ??
• ???????
• ????????????
• ??????????
• ????
• ?????????
• ??????????
• ??????
• ?????
• ??????

• ??
• ??????
• ( ?????? ????)
• ?????????????
• (????)
• ??????????IMViC????
• (?????? ?????)

2
Nutrient requirements of bacterial growth

• water
• Carbon source
• Nitrogen source
• Minerals
• Growth factors
• organic compounds such as amino acids,
  purines??, pyrimidines??, and vitamins that a
  cell must have for growth but cannot synthesize
  itself.

3

• culture medium??? Substances used for
  artificial cultivation of bacteria.

4

• Classification of culture medium
• According to basic ingredients
• Minimal essential growth medium?????
• enrichment medium?????
• selective medium?????
• differential medium?????
• According to physical condition
• liquid medium?????
• solid medium????? 1.52.5 of agar
• semisolid medium?????? 0.30.5 of agar

5
Phenomena of bacterial growth????????????

• In liquid medium surface growth pellicle????,
  uniformly turbid??????, sediment in bottom????

6

• On solid medium

7

• In semisolid medium

??? ???
8
susceptibility test ????
?
?
?
9
1
2
3
10
uv30
???
?????/????? ??
11
(No Transcript)
12
?????
?????
IMViC?? I - ??(indol)?? M - ???(methyl
red)?? V - VP(Voges-Proskauer)?? C
-??????(citrate utilization)??