??? (mono-), ??? (di-), ??? (tri-) ? ??? (poly-saccharides) - PowerPoint PPT Presentation

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??? (mono-), ??? (di-), ??? (tri-) ? ??? (poly-saccharides)

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Title: III Author: Song Moon Jung Last modified by: park Created Date: 9/23/2004 2:07:24 AM Document presentation format – PowerPoint PPT presentation

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Title: ??? (mono-), ??? (di-), ??? (tri-) ? ??? (poly-saccharides)


1
??? ???? ?? III
  • 2006. 9. 21.-22.
  • ???? ? ? ?
  • ???? ? ? ?

2
??? ???? ??
3
??
  • ??? ???? ? ????, ??? ??? ?? ??? ???? ? ??? ??? ?
    ??? ??.
  • ??? ??? ??? ???? ? ??? ??? ? ??? ??.

4
????
  • ??? ??? ? ???????? ??
  • ??
  • ??? (mono-), ??? (di-), ??? (tri-) ? ???
    (poly-saccharides)
  • ????
  • Acids (acetic acid, lactic acid) Gas (??, ??)
  • ?? ??? ?? ???? ??? ??? ???, ???? ??? ??? ?? ???
    ??
  • ? ??? ????? ??
  • ????? ?? ?? ??
  • ??? ??
  • ??? ?? ??
  • Methyl-Red ? Voges-Proskauer (MR-VP) ??

5
??? ??
  • ?? Bacilus subtilis, E.coli,
  • ?? phenol red? ??? glucose ?? lactose broth?
    ???? Durham tube? ? ?? (loop??)
  • Phenol red ???, pK 7.0
  • Yellow (pH6.8) ? ? (pH 8.4) Red
  • ?? ?? ?? ???? ?? ?? ??? ????? ?? ??

Glucose Lactose Sucrose
B. subtilis
E. coli
S. Paratyphi A
Control (-)
6
??? ??
  • ?? Bacilus subtilis, E.coli,
  • ?? phenol red? ??? glucose ?? lactose ??? ????
    Durham tube? ? ??
  • Phenol red ???
  • Yellow (pH6.8) ? ? (pH 8.4) Red
  • ?? ?? ?? ???? ?? ?? ??? ????? ?? ??

Glucose Lactose Sucrose
B. subtilis
E. coli /gas /gas
S. Paratyphi A /gas
Control(-)
7
IMViC test
I Indole production M Methyl Red Vi
Voges-Proskauer test C Citrate utilization test
Escherichia ? Enterobacter ? ??
8
??? ?? ?? (Citrate Utilization Test)
  • ?? Escherichia coli, Enterobacter aerogenes
  • ?? Simmons citrate agar slant (????? ??? ??????
    ??????)? ? ?? (needle ??)
  • ??? bromothymol blue
  • Yellow (pH 6.2) ? ? (pH 7.6) Blue
  • ?? ?? ???? ??? ???? ??
  • ???? ?? green? blue
  • ????? ????

9
Bromothymol blue, also known as bromthymol blue,
is a chemical indicator for weak acids and bases.
Following are given its colours at different
solution pH values. Alkaline (basic) - Blue (pH
gt 7) Neutral - Green (pH 7) Acid - Yellow (pH
lt 7) The bromothymol blue acts as a weak acid in
solution and therefore can be in acid or base
forms which appear yellow and blue respectively
C21H15NaBr2O3S ? H C21H14NaBr2O3S- The pKa
for bromothymol blue is 7. Formula
C21H15NaBr2O3SAlternative name
Dibromothymolsulfonephthalein, BTB
10
Methyl-Red and Voges Proskauer test (MR-VP test)
Enteric group bacteria Gram negative
Facultative anaerobiosis
1. Escherichia,
Salmonella, Shigella
coliform bacteria
2. Enterobacter, Serratia, Erwinia
3. Proteus,
Providencia 4.
Yersinia
11
Mixed-acid fermentation
Butanediol fermentation
Acetoin (acethyl-methyl-carbinol) ??
12
(No Transcript)
13
  • ?? E. coli, E. aerogenes
  • ??
  • ?? MR-VP media broth 2?? MR?, 2?? VP?
  • MR test ?? test
  • ??? ?? ???? ?? ??? pH? ???
  • Methyl Red Red (pH 4.2) ? ? (pH6.3) Yellow
  • VP test acetoin (acethyl-methyl-carbinol) ????
  • pH? ??? ??? VP ??? ???? ?? ?? acetoin? ??
  • VP?? A (5 ?-naphthol, 8-10 drops) ??? ??
  • VP?? B (0.3 creatine in 40 KOH, 8-10 drops) ???
    ??
  • RT, 20?
  • ???? pink? red

14
???? ????? ?? ??
  • Indole ?? ?? tryptophan hydrolysis? ??? indole??
    ??
  • - Tryptophanase ? ?? ??
  • ???? ?? ?? (KIA slant, Kliglers Iron Agar)
  • H2S Fe ? FeS (Black) H2 gas
  • Sulfur? ???? amino acid? ???? H2S gas ??? H2S
    gas? Fe?????? FeS ??
  • FeS ??? ?? H2S? ??? ??

15
Indole ????
  • ? B. subtilis, E. coli
  • ?? tryphtopahn broth? ??? ?? (loop??)
  • ?? ??? Kovac?? (2-3 drops) ? alchol?? ??? ??
    ??? indole??
  • ?? Kovac??- HCl ??

16
(No Transcript)
17
???? ?? ??
  • ?? E. coli, S. paratyphi A, Proteus vulgaris
  • ?? KIA slant (Fe ?? ??)? ? ?? (needle??)
  • ?? ??? ?? ?? ??

18
??? ??
  • ??? ????? (Semisolid agar method, 0.2-0.4 ??)
  • ?? B. subtilis, P. vulgaris ? ??- ?? streaking?
    ?? ?? ??? ???? ?.
  • ?? ??? ?? ?? ??

19
????? ??? ??? ??
20
??
  • ??? ??? ????? ????? ??? ??? ??? ? ??? ??
  • ? ? ???? ????? ??? ??? ??? ? ??? ??

21
?? ?? ??? ???
  • ?? B. subtilis (18h, 72h), E. coli (18h)
  • ?? ????? ??? ?? ?? 60?, 80?? water bath?? 10??
    ????? ? ???? ??
  • Spore?? ??? ?? ??

22
(No Transcript)
23
???? ????? ??? ??
  • ?? S. aureus, E. coli
  • ?? Nutrient agar (0.85 ?? 7.5 NaCl ??)? ??,
    ???? ??
  • Plate? ?? ??? ??? ?? ??? ? (labeling ??)
  • ????? 0.5-3? ????? ??
  • ???? ???? ?? V. parahaemolyticus

24
??? ??? ??
25
??
  • ??? ??? ????? ???? ? ??? ??? ? ??? ??
  • ?? ???? ????? ???? ???? ?? ??? ??? ? ??? ??
  • ????????? ??????? ??? ? ??? ??
  • ??? ????? ??? ??? ??? ????? ??? ????? ??

26
??? ??? ????
  • ??? ???
  • ????? (Kirby-Bauer method)
  • ?? ??? (??/disc)
  • Carbenicillin (100 ?)
  • Cephaolthin (30 ?)
  • Chloramphenicol (30 ?)
  • Erythromycin (15 ?)
  • Gentamycin (10 ?)
  • Penicillin (10 unit)
  • Tetracycline (30 ?)

27
?? ???
  • ?? S.aureus, E.coli
  • ??
  • ?????? ??? ? ??? ???? Muller-Hinton agar??? ?
    ????? (? ??? 2 plates)
  • ? ??? disc? plate? ??? ???. (3-4?? disc/plate)

28
(No Transcript)
29
??? ??? ??? ?? ? ??? ?? ??? ?
30
(No Transcript)
31
(No Transcript)
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