Title: ??? (mono-), ??? (di-), ??? (tri-) ? ??? (poly-saccharides)
1??? ???? ?? III
- 2006. 9. 21.-22.
- ???? ? ? ?
- ???? ? ? ?
2??? ???? ??
3??
- ??? ???? ? ????, ??? ??? ?? ??? ???? ? ??? ??? ?
??? ??. - ??? ??? ??? ???? ? ??? ??? ? ??? ??.
4????
- ??? ??? ? ???????? ??
- ??
- ??? (mono-), ??? (di-), ??? (tri-) ? ???
(poly-saccharides) - ????
- Acids (acetic acid, lactic acid) Gas (??, ??)
- ?? ??? ?? ???? ??? ??? ???, ???? ??? ??? ?? ???
?? - ? ??? ????? ??
- ????? ?? ?? ??
- ??? ??
- ??? ?? ??
- Methyl-Red ? Voges-Proskauer (MR-VP) ??
5??? ??
- ?? Bacilus subtilis, E.coli,
- ?? phenol red? ??? glucose ?? lactose broth?
???? Durham tube? ? ?? (loop??) - Phenol red ???, pK 7.0
- Yellow (pH6.8) ? ? (pH 8.4) Red
- ?? ?? ?? ???? ?? ?? ??? ????? ?? ??
Glucose Lactose Sucrose
B. subtilis
E. coli
S. Paratyphi A
Control (-)
6??? ??
- ?? Bacilus subtilis, E.coli,
- ?? phenol red? ??? glucose ?? lactose ??? ????
Durham tube? ? ?? - Phenol red ???
- Yellow (pH6.8) ? ? (pH 8.4) Red
- ?? ?? ?? ???? ?? ?? ??? ????? ?? ??
Glucose Lactose Sucrose
B. subtilis
E. coli /gas /gas
S. Paratyphi A /gas
Control(-)
7IMViC test
I Indole production M Methyl Red Vi
Voges-Proskauer test C Citrate utilization test
Escherichia ? Enterobacter ? ??
8??? ?? ?? (Citrate Utilization Test)
- ?? Escherichia coli, Enterobacter aerogenes
- ?? Simmons citrate agar slant (????? ??? ??????
??????)? ? ?? (needle ??) - ??? bromothymol blue
- Yellow (pH 6.2) ? ? (pH 7.6) Blue
- ?? ?? ???? ??? ???? ??
- ???? ?? green? blue
- ????? ????
9Bromothymol blue, also known as bromthymol blue,
is a chemical indicator for weak acids and bases.
Following are given its colours at different
solution pH values. Alkaline (basic) - Blue (pH
gt 7) Neutral - Green (pH 7) Acid - Yellow (pH
lt 7) The bromothymol blue acts as a weak acid in
solution and therefore can be in acid or base
forms which appear yellow and blue respectively
C21H15NaBr2O3S ? H C21H14NaBr2O3S- The pKa
for bromothymol blue is 7. Formula
C21H15NaBr2O3SAlternative name
Dibromothymolsulfonephthalein, BTB
10Methyl-Red and Voges Proskauer test (MR-VP test)
Enteric group bacteria Gram negative
Facultative anaerobiosis
1. Escherichia,
Salmonella, Shigella
coliform bacteria
2. Enterobacter, Serratia, Erwinia
3. Proteus,
Providencia 4.
Yersinia
11Mixed-acid fermentation
Butanediol fermentation
Acetoin (acethyl-methyl-carbinol) ??
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13- ?? E. coli, E. aerogenes
- ??
- ?? MR-VP media broth 2?? MR?, 2?? VP?
- MR test ?? test
- ??? ?? ???? ?? ??? pH? ???
- Methyl Red Red (pH 4.2) ? ? (pH6.3) Yellow
- VP test acetoin (acethyl-methyl-carbinol) ????
- pH? ??? ??? VP ??? ???? ?? ?? acetoin? ??
- VP?? A (5 ?-naphthol, 8-10 drops) ??? ??
- VP?? B (0.3 creatine in 40 KOH, 8-10 drops) ???
?? - RT, 20?
- ???? pink? red
14???? ????? ?? ??
- Indole ?? ?? tryptophan hydrolysis? ??? indole??
?? - - Tryptophanase ? ?? ??
- ???? ?? ?? (KIA slant, Kliglers Iron Agar)
- H2S Fe ? FeS (Black) H2 gas
- Sulfur? ???? amino acid? ???? H2S gas ??? H2S
gas? Fe?????? FeS ?? - FeS ??? ?? H2S? ??? ??
15Indole ????
- ? B. subtilis, E. coli
- ?? tryphtopahn broth? ??? ?? (loop??)
- ?? ??? Kovac?? (2-3 drops) ? alchol?? ??? ??
??? indole?? - ?? Kovac??- HCl ??
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17???? ?? ??
- ?? E. coli, S. paratyphi A, Proteus vulgaris
- ?? KIA slant (Fe ?? ??)? ? ?? (needle??)
- ?? ??? ?? ?? ??
18??? ??
- ??? ????? (Semisolid agar method, 0.2-0.4 ??)
- ?? B. subtilis, P. vulgaris ? ??- ?? streaking?
?? ?? ??? ???? ?. - ?? ??? ?? ?? ??
19????? ??? ??? ??
20??
- ??? ??? ????? ????? ??? ??? ??? ? ??? ??
- ? ? ???? ????? ??? ??? ??? ? ??? ??
21?? ?? ??? ???
- ?? B. subtilis (18h, 72h), E. coli (18h)
- ?? ????? ??? ?? ?? 60?, 80?? water bath?? 10??
????? ? ???? ?? - Spore?? ??? ?? ??
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23???? ????? ??? ??
- ?? S. aureus, E. coli
- ?? Nutrient agar (0.85 ?? 7.5 NaCl ??)? ??,
???? ?? - Plate? ?? ??? ??? ?? ??? ? (labeling ??)
- ????? 0.5-3? ????? ??
- ???? ???? ?? V. parahaemolyticus
24??? ??? ??
25??
- ??? ??? ????? ???? ? ??? ??? ? ??? ??
- ?? ???? ????? ???? ???? ?? ??? ??? ? ??? ??
- ????????? ??????? ??? ? ??? ??
- ??? ????? ??? ??? ??? ????? ??? ????? ??
26??? ??? ????
- ??? ???
- ????? (Kirby-Bauer method)
- ?? ??? (??/disc)
- Carbenicillin (100 ?)
- Cephaolthin (30 ?)
- Chloramphenicol (30 ?)
- Erythromycin (15 ?)
- Gentamycin (10 ?)
- Penicillin (10 unit)
- Tetracycline (30 ?)
27?? ???
- ?? S.aureus, E.coli
- ??
- ?????? ??? ? ??? ???? Muller-Hinton agar??? ?
????? (? ??? 2 plates) - ? ??? disc? plate? ??? ???. (3-4?? disc/plate)
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29??? ??? ??? ?? ? ??? ?? ??? ?
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