Apresentao do PowerPoint

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Effect of ErYAG laser irradiation on the
dentinal diffusion of Calcitonin and Sodium
Alendronate labelled with Iodine 131 and
Technetium 99.
Marina Stella Bello Silva Eduardo, C. P. Apel,
C. Gutknecht, N Lage-Marques, J. L. DAVID SCOOT
FELLOWSHOP IADR Brasil - 2005
IPEN - USP
Uniklinikum Aachen-RWTH
Dentistry Faculty USP
LELO - USP
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Purpose
The aim of this study is to evaluate the
influence of ErYAG laser on Calcitonin and
Alendronate diffusion through dentin
tubules. These medicaments are being tested as
filling material in order to treat external root
resorption that frequently occurs after dental
trauma.
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Root Resorption
Substitutive resorption and Ankylosis
Inflammatory resorption
Traumatized tooth
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Treatment
Conventional Endodontic Treatment
Specific intracanal filling able to diffuse
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Calcitonin and Sodium Alendronate

• Medicaments used to treat osteoporosis
• Prevent and treat hard tissues resorption

?
Calcitonin
Hormone
TESTED AND USED IN DENTISTRY
?
Alendronate
Derived from pyrophosphate acid
MORE STUDIES NEEDED
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Radioisotopes

• Better evaluation ofpermeation
• More reliability

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Laser and Diffusion
Not irradiated surface Presence of smear layer
Irradiated surface Opened tubules
AALZ
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Methods
40 teeth
160 samples
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Methods
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Groups
G1 131I Calcitonin G2 99Tc Alendronate
No treatment received
Treated for 10 min in EDTA-T and next for 15 min
in Sodium Hypochlorite Irradiated by ErYAG
laser
G1T 131I Calcitonin G2T 99Tc Alendronate
G1TI 131I Calcitonin G2TI 99Tc Alendronate
G1I 131I Calcitonin G2I 99Tc Alendronate
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ErYAG Kavo Key III LaserExchange between Kavo,
Germany, and LELO FOUSP, Brazil.
Type 2065Tip 50/10

• Thickness 0.47 mm
• Energy at the distalend 54 mJ
• Energy density of57.6 J/cm2

• Wavelenght of 2.94 µm
• Pulse energy 100 mJ on display
• Repetition rate 6 Hz
• Endodontic beam perpendicular and5 mm away from
  moist sample surface
• 4 applications, 10 seconds each

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Methods
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Methods
Margin impermeabilization
10 µl of labelled Calcitonin solution 10 µl of
labelled Alendronate solution 4 hours
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Methods
Removing top and remaining solution
Not diffused medicament
Vial with solution and piece of paper
Wax and dentin specimen
Diffused medicament
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Analysis
Packard Gama Counter Kruskal-Wallis
statistical analysis
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Results
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Conclusion
According to the results obtained in this
study, the groups treated with EDTA-T and
Hypochlorite and irradiated by ErYAG laser
presented higher rates of Calcitonin and
Alendronate diffusion. When comparing diffusion
of both medicaments, Calcitonin presented higher
rates than Alendronate, in all groups analysed.
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Special thanks to

Prof. Dr. Norbert Gutknecht Dr. Christian
Apel Dipl.-Bibl. Leon Vanweersch Dr. Jörg
Meister Dr. René Franzen Dr. Maziar Mir
Prof. Dr. Carlos de Paula Eduardo Prof. Dr. José
Luiz Lage-Marques Prof. Dr. Elaine B.
Araújo Prof. Dr. Constancia P. G. Silva Dr. Maria
Tereza Colturato Josefina S. Santos
Alyne Simões Juliana Marotti Leila S.
Ferreira Vanessa L. Freitas
LELO and IPEN Team Kavo, Germany
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Marina Stella Bello Silva marinasbello_at_yahoo.com.b
r
VIELEN DANK!
Fernando de Noronha - Brasil