Apresentao do PowerPoint

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Malaria and Dengue A mosquito-based approach
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• Design anti-pathogen genes

• Mosquito Population

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Culex quinquefasciatus
West Nile fever
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An. darlingi
An. aquasalis
Malaria
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Ae. aegypti Ae. albopictus
Dengue fever
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Anti-pathogen genes

• Recombinant antibodies
• (scFv - single chain antibody)

• Active peptides

• Protein domains

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• Recombinant antibodies
• (scFv - single chain antibody)

1A10-2 1B7 E protein 3H5 9A3D-8 2H2
prM
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1A10-2-scFv
Sindbis expression system
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pBac3xP3-EGFP, Vg-effector
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Plasmodium gallinaceum and Aedes aegypti
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Transgene
Effectors Molecules
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Aedes aegypti fat body transcriptome
50.82
30.05
5.16
7.97
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Macro array
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130 transcripts
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Proteomic approach on hemolymph proteins
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BF
Sucrose
24 hs
OV
48 hs
72 hs
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Non-infected P.gal infected
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Mosquitoes Population
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José Eduardo Bracco e Dr. Maria Anice Sallun
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Uganda, Singapore, Cambodia and Tahiti
Senegal
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The haplotype network for mitochondrial ND4 from
Aedes aegypti populations from Brazil. Each line
in the network represents a single mutational
change. White circle represents a theoretical
interior node.
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Ae. albopictus
Ae. aegypti
Specie-specific markers
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• Dr. José Eduardo Levi
• IMT - USP

Dr. Margareth L. Capurro ICB USP Bianca
Burini Fernanda Rosa MT - FAPESP Ricardo V.
Araújo DD - CNPq Fabiana M. Feitosa DD -
FAPESP JoãoV. Camargo e Silva DD -
CAPES Daniela G. Rodrigues MT - CNPq Ceres M.
de Miranda MT - CNPq Márcia Sperança PosDoc -
FAPEMA José Eduardo Bracco DT-
(SUCEN) Alexandre Moura technical support

• Dr. Anthony A. James
• UCI

• Dr. Ken Olson
• Colorado State

• Dr. Maria Anice Sallun
• FSP - USP

• Dr. Tania Bijovsky
• ICB - USP

Supported by FAPESP