Antibody Design & Conjugation

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Antibody Design Conjugation
https//www.creative-biolabs.com/adc
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To achieve a narrower DAR, Creative Biolabs has
explored and adopted a variety of methodologies
in antibody design and engineering to introduce a
series of specific and chemically versatile
conjugation sites into the antibody sequences

• We incorporate Cysteine residues into defined
  sites on an antibody to create thiol-engineered
  antibody (EnCys-mAb) and conjugate payload drugs
  onto those Cysteine residues via thiol-based
  chemistry.
• Unnatural amino acids (UAAs), such as p-Acetyl
  Phenylalanine, are integrated into antibody
  sequences via amber stop codon-mediated
  transcription and their chemically diverse side
  chains are excellent sites to introduce the
  payload drug for ADC preparation.

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• 3. Some enzymes, such as Sortase A,
  transglutaminases, formylglycin generating
  enzyme, recognize and modify certain amino acids
  on a peptide motif to yield chemically activated
  side chains. Creative Biolabs exploits these
  approaches and developed Enzyme-mediated
  modification and conjugation system to help
  generate ADCs with specific conjugation sites.
• 4. Through antibody design, we create a cave on
  the desired locations on an antibody to
  facilitate meditope trapping and subsequently,
  achieve site-specific meditope-based
  conjugations.
• 5. We take advantage of the self-splicing inteins
  and engineer intein-fusion antibodies, thereby
  achieving C-terminal specific antibody
  modification and yielding conjugates with DARs at
  1 or 2.

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ADC Antibody

• As one major component of an antibody-drug
  conjugate (ADC), the antibody is the key for
  target specificity and serves as the cargo to
  deliver the cytotoxic drug (payload). A payload
  drug can be attached to different sites on an
  antibody using diverse conjugation chemistry.

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Antibody Conjugates
Scientists at Creative Biolabs are experienced
in performing Lysine conjugation (amine reaction)
as well as Cysteine conjugation (thiol reactions)
to create ADCs using these endogenous sites. We
are also expertise in exploiting Tyrosine
conjugation approaches as another innovated
endogens site for ADC development.
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Some endogenous or engineered sites for the
conjugation of cytotoxic payload drugs to an
antibody (Antibodies,2015).
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Antibody Conjugation service
Designing a chemically compatible antibody for
the conjugation chemistry is a key step that
directly dictates the fate of an ADC project.
With expertise and dedication, advanced antibody
design platform and services from Creative
Biolabs will be your best companion in creating
customized ADCs. Please contact us for more
information and a detailed quote.
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45-1 Ramsey Road, Shirley, NY 11967, USA Email
marketing_at_creative-biolabs.com
Contact us
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