Introducing Betty and Bob

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Introducing - Betty and Bob
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Once Upon a time

• There was Betty
• Betty was beautiful
• Some say she was a babe
• Betty the beautiful babe

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Then there was Bob

• Bob was big
• but bashful
• Big bashful Bob

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• Bob was lonely
• Very lonely

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Then one day

• Bob saw Betty At Bens Bar
• Their eyes met
• It was love

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Soon Betty and Bob Were United In Wedded Bliss
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And then came along baby Bart

• Bart was a bouncing baby boy
• Being Bob and Bettys boy, of course he was big,
• big and bouncing,
• a big bouncing baby boy!

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Betty and Bob were so happy
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Two years later.
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Betty said, Bob, Were going to have another
baby!
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Soon Baby Billy Was Born

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Bob was bothered

• Billy didnt look like Bart
• And didnt look like Bob
• He looked more like the neighbor.

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BRAD
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Bob was MAD

• (CANT YOU JUST FEEL THE TENSION?)

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They packed up the family and went to the local
genetic testing facility and met
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Dr. Doug

• Who was delightful,
• Debonair,
• Dashing,
• A real dude
• And knew about
• DNA

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Dr. Doug did what DNA dudes do(this is where
you start taking notes)

• 1. Took a sample of DNA from each individual
• 2. Cut the DNA with
• restriction enzymes

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So, what the heck (a Utah word) is a restriction
enzyme???
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Enzymes that

• Recognize specific nucleotide sequences
• Then Cuts the DNA at those sites

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Example of a restriction enzyme EcoRI

• EcoRI is an enzyme that recognizes the GAATTC
  sequence of nitrogenous bases
• EcoRI would make the cut like this
• CAGAAT TCAT GTC TTAAG TA

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And would leave us with two pieces of DNA, called
fragments

• CAG AAT TCAT GTC TTAA GTA

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You try this one

• The enzyme PstI recognizes the sequence C T G C
  A G (PSTI will make the cut between the A G)
• AAT CCTGCAGCT ACTGCAGTTA
• TTA GGACGTCGTAGACGTCAAT
• How many fragments?

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• AAT CCTGCAGCT ACTGCAGTTA
• TTA GGACGTCGTAGACGTCAAT

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Bob and Betty Wait

• Oh yea. Bob and Betty

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3. The DNA (with the restriction enzyme) is
mixed with a dye
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4. The gel is loaded into an electrophoresis
chamber
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5. A buffer solution covers the gel
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6.The DNA is loaded onto an agarose gel
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Betty and Bob are still waiting.
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7. An electrical current is run through the
chamber

• DNA has a negative charge
• The DNA will migrate through the gel towards the
  positive end

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8. The gel is stained then dried

• Staining is necessary in order to see the bands
  of DNA

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Lets Take a look at The Process

• A quick simulation

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Now we are ready to evaluate our gel
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Ok Betty. Lets see what happened?
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Gel

• Mmom
• C Billy
• F1Brad
• F2 Bob

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What do you think? Who is the father? Bob or Brad?

• F1Brad
• F2Bob

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Bob looks at Betty
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Betty looks at Bob
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They smile
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Oh, I love you Betty..
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Oh, I love you Brad d d d
I mean BOB!
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Works Cited and Consulted

• None