CHAPTER 2 The Chemistry of Living Things

1

• Bioethics 7pm Thursday?
• Go or read an article and come prepared to
  discuss it.

2

• Lox Recombination
• Present the HA tag at carboxy end.

3

• Bar Coding Mutant Strains Fig 6.7-6.8

4

• Old school antisense
• New schoolRNA interference

5

• Protein Protein interactions
• CoIP or Affinity purification
• Two Hybrid 6.14-15
• AD and BD

6

• RNA interference

7

• Determining Structures-Structural Proteomics
• Figure 6.10 Rational Approach

8

• Aquaporin as an example Fig 6.11
• How to prevent H30 entry?
• Homotetramer 3A, Water is 2.8 A

9

• Structure Based or Rational
• Drug Design
• One nice trick use crystallization Fig 6.12

10

• 2-D Gels Figure 6.21