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Biochem Open Note Quiz

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7. What do the following reagents test for: Iodine, Sudan III and Biurets Solution. ... Test for using Biuret's. Turns violet if proteins. Protein Structure ... – PowerPoint PPT presentation

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Title: Biochem Open Note Quiz


1
Biochem Open Note Quiz
  • 1. What is an Atom? What is an element? What
    determines the chemical properties of an element?
  • 2. Draw the structure of Boron (Atomic 5).
    Show how many protons, neutrons and electrons it
    has. Make sure to follow the Octet Rule, placing
    electrons properly.
  • 3. Bleach has a pH of 13. Is it acidic or
    basic? What type of ions? Vinegar has a pH of
    4. Is it acidic or basic? What type of ions?
  • 4. What is a monomer? What is a polymer? What
    are the 4 macromolecules? What are
    macromolecules used for?
  • 5. Why can so many water droplets fit on the
    face of a coin? Why does ice float?
  • 6. Take a piece of candy from the jarwhat
    macromolecule are you consuming?
  • 7. What do the following reagents test for
    Iodine, Sudan III and Biurets Solution. How do
    you know if you have a positive or negative test
    for each?

2
Biochem Unit Review
  • Basic Chemistry
  • Acids and Bases
  • Water
  • Macromolecules

3
Atomic Structure
  • Atom protons, neutrons, electrons
  • Atomic protons (usually of electrons and
    neutrons)
  • Isotope different neutrons than Atomic
  • Ion different of electrons than Atomic

4
Energy levels and bonding
  • If outer orbital (valence shell) is not full of
    electrons, an atom has 3 choices
  • give up extra ones to fall down to the next
    level,
  • gain more to fill up the current level,
  • share what they have with another atom.
  • Loss or gain results in ions (charged atoms)
    opposite charges attract - ionic bonds
  • Wimpy Sodium (Na) and Big Bad Chlorine (Cl)
  • Sharing of electrons - covalent bonds (H-H)

5
Chemical reactions/Bonding trying to reach
chemical equilibrium
6
Hydrogen Bonding
  • Weak attractions between an electronegative (-)
    atom and a hydrogen ()
  • Water wimpy H() and big bad O(-)
  • Individually, hydrogen bonds break easily
    collectively, they work to stabilize structure.

7
Elements, Molecules and Compounds
  • Element basic unit of chemistry properties
    based on of protons
  • H, C, N, O, P, S
  • Compounds chemical bonding of different
    elements
  • H2O NaCl, HCl

8
pH Scale
  • The pH Scale is a system designed by scientist to
    measure the amount of hydrogen ions (H) or
    hydroxide ions (OH-) in solution.
  • The scale ranges from 0 to 14, with 7 being
    neutral or an equal number of H and OH-. Pure
    water is neutral allowing it to be a component of
    even really strong acids and bases.

NEXT
9
Acids
  • Acids are solutions that have a higher
    concentration of H ions and whose pH value
    falls below 7.
  • Lemons vinegar Battery Acid

10
Bases
  • Bases are solutions that have a higher
    concentration of OH- ions and whose pH value is
    above 7.
  • Soaps and cleaners slippery feel
  • NaOH

11
Physiology of pH change in the Body
12
Organic Macromolecules
  • Carbohydrates
  • Lipids
  • Proteins
  • Nucleic Acids

A macromolecule is a larger molecule (polymer)
built by putting together smaller single units
(monomers).
13
Carbohydrates fuel and building material
  • Three types
  • 1. mono-saccharides
  • 2. di-saccharides
  • 3. poly-saccharides
  • Made up of C, H, and O
  • Number of sugar molecules attached determines
    category
  • mono- one
  • di- two
  • poly- more than two
  • Glycosidic bonds attach the sugar monomers
    together

14
Chemical Identification of Carbohydrates
  • Benedicts Test Identifies the presence of a
    MONOSACCHARIDE by changing from blue to orange in
    the presence of heat not done in class
  • Iodine Test Identifies the presence of a
    POLYSACCHARIDE by changing from yellow to
    purple/black done in CSI lab(starch)
  • Process of Elimination If there is no reaction
    with either the Benedicts or Iodine Tests, then
    a DISACCHARIDE is present.

15
Lipid Structure
  • Lipid molecules are made up of fatty acids and
    glycerol
  • Energy storing, cushioning and insulation
  • Unsaturated fat- a fat that contains at least one
    carbon-carbon double bond (it is liquid at room
    temperature) less H
  • Saturated fat- a fat without one carbon-carbon
    double bond (solid at room temperature)
  • Test for using Sudan III

16
Nucleic Acid StructureRNA and DNA polymers of
information
  • Nucleic Acids are made up of nucleotides bonded
    together
  • Nucleotides consist of 3 parts
  • 5-carbon sugar
  • A phosphate group
  • And a nitrogenous base

17
Proteins
  • Proteins control the rate of reactions and
    regulate cell processes. (enzymes are proteins)
  • Proteins are used to form bones and muscles
  • Proteins transport substances into or out of
    cells or help to fight disease
  • Test for using Biurets
  • Turns violet if proteins

18
  • Protein Structure
  • (1)Primary Structure- amino acid sequence in a
    polypeptide chain
  • (2)Secondary Structure- polypeptide chains
    hydrogen bonded into a helix form
  • (3)Tertiary Structure- one complete protein chain
    clumped up
  • (4)Quaternary Structure- many protein lumps stuck
    together

19
Chemical Reactions and Enzymes
  • Activation energy- energy needed to get a
    reaction started
  • Enzymes are proteins that act as biological
    catalysts (speed up a reaction)

20
Carbon Macromolecules Compounds
include
that consist of
that consist of
that consist of
that consist of
which contain
which contain
which contain
which contain
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