Cell Lineage of C' elegans

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Cell Lineage of C. elegans
Where do we come from? Where are we going? When?
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Early Embryonic Development4D Microscopy
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The Good, the Bad, and the Uncly
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So Why Map Genes?
1. Insure phenotype is caused by a single
mutation.
2. Reduce number of complementation tests.
3. Facilitate genetic epistasis
analysis (construction of double mutants (genetic
epistasis analysis will be explained in the next
lecture).
4. Identify cases (rare) of non-allelic
non- Complementation (rarely mutations That are
not alleles will fail to complement).
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Worm Microinjection The Needle and the Damage
Done
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Analysis of Gene Expression in Living Animals
Green Flourescent Protein (GFP)
238 amino acids
absorbs 395 nm emits 508 nm
Requires no cofactor
Active in all cells and cellular compartments
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