The nematode C' elegans

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The nematode C. elegans

• all cell lineages mapped from zygote to death
  (1980)
• fully sequenced genome (1998)
• fast transient transformation
• reporters for gene expression analyses
• RNA interference by injection, soaking, feeding
  (1998)

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Caenorhabditis elegans
Less than 1000 cells in adult. Self-fertilizing
hermaphrodite Short generation time
Sydney Brenner
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Early cell lineage in C. elegans
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Reverse genetics in C. elegans

• fast transient transformation not an
  integrated transposon but an extrachromosomal DNA
  array
• reporters for gene expression analyses
• RNA interference by injection, soaking, feeding

Analyze tissue- and cell-specific gene expression
in vivo Create GOF phenotypes, demonstrate
tissue-specific roles of genes
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Gonad cells development
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Feeding RNAi